Sandbox 148: Difference between revisions
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== How does Kapβ2 identify its cargo? == | == How does Kapβ2 identify its cargo? == | ||
The NLS located on Kapβ2 cargos, are named the PY-NLS and they bind to the C-terminal arch of Kapβ2. The internal surface of kapβ2 superhelix at the C-terminal arch has negative electrostatic potential. The recognition of the <scene name='37/372748/Kapb2_rainbow_nls/1'>PY-NLS</scene> by Kapβ2 follows certain guidelines: (1) PY-NLS, when not bound to Kapβ2, lacks a secondary structure; (2) PY-NLS has an overall positive charge allowing for electrostatic compatibility with Kapβ2; (3) the general sequence for PY-NLS is either a hydrophobic or a basic motif at the N-terminus and a R-X<sub>2-5</sub>P-Y motif at the C-terminus. | The NLS located on Kapβ2 cargos, are named the [https://en.wikipedia.org/wiki/Nuclear_localization_sequence PY-NLS] and they bind to the C-terminal arch of Kapβ2. The internal surface of kapβ2 superhelix at the C-terminal arch has negative electrostatic potential. The recognition of the <scene name='37/372748/Kapb2_rainbow_nls/1'>PY-NLS</scene> by Kapβ2 follows certain guidelines: (1) PY-NLS, when not bound to Kapβ2, lacks a secondary structure; (2) PY-NLS has an overall positive charge allowing for electrostatic compatibility with Kapβ2; (3) the general sequence for PY-NLS is either a hydrophobic or a basic motif at the N-terminus and a R-X<sub>2-5</sub>P-Y motif at the C-terminus. | ||
The PY-NLS of 4OO6 contains a basic rather than a hydrophobic N-terminal motif. Basic interactions at the N-terminal motif pf the PY-NLS include: Arg 92, Arg 94 and Arg 96 of the NLS with Glu 588 and Glu 496 of Kapβ2. Interactions of the C-terminal R-X<sub>2-5</sub>P-Y motif of the NLS include: Pro 98 of the NLS with Ile 456 and Trp 459 of Kapβ2. | The PY-NLS of 4OO6 contains a basic rather than a hydrophobic N-terminal motif. Basic interactions at the N-terminal motif pf the PY-NLS include: Arg 92, Arg 94 and Arg 96 of the NLS with Glu 588 and Glu 496 of Kapβ2. Interactions of the C-terminal R-X<sub>2-5</sub>P-Y motif of the NLS include: Pro 98 of the NLS with Ile 456 and Trp 459 of Kapβ2. | ||