Purine repressor: Difference between revisions
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<StructureSection load=' | <StructureSection load='1wet' size='350' side='right' caption='Structure of E. coli purine processor complex with DNA (green) and guanine (PDB entry [[1wet]])' scene=''> | ||
== Function == | == Function == | ||
'''Purine repressor''' (PurR) is a member of the lac repressor family. PurP binds DNA via a highly conserved helix-turn-helix at the N terminal (DBD). PurP contains a co-repressor binding domain as well (CBD). PurP binds to a 16-bp operator sequence and co-regulates genes which are involved in the biosynthesis of purine and pyrimidine nucleotides<ref>PMID:1971621</ref>. | '''Purine repressor''' (PurR) is a member of the lac repressor family. PurP binds DNA via a highly conserved helix-turn-helix at the N terminal (DBD). PurP contains a nucleotide co-repressor binding domain as well (CBD). PurP binds to a 16-bp operator sequence and co-regulates genes which are involved in the biosynthesis of purine and pyrimidine nucleotides<ref>PMID:1971621</ref>. | ||
== Structural highlights == | |||
The PurP guanine co-repressor binding site includes stacking interactions as well as hydrogen bonded ones. The DNA binding domain contains a helix-turn-helix-loop-helix motif which interacts with the DNA major groove and a hinge helix binding to to the DNA minor groove. Residue L53 interdigitates with the DNA central base pair<ref>PMID:9278422</ref>. | |||
</StructureSection> | </StructureSection> | ||