4z45: Difference between revisions
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<table><tr><td colspan='2'>[[4z45]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z45 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z45 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4z45]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z45 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z45 FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4z39|4z39]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4z39|4z39]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z45 OCA], [http://pdbe.org/4z45 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z45 RCSB], [http://www.ebi.ac.uk/pdbsum/4z45 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z45 OCA], [http://pdbe.org/4z45 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z45 RCSB], [http://www.ebi.ac.uk/pdbsum/4z45 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z45 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Aphids use chemical cues to locate hosts and find mates. The vetch aphid Megoura viciae feeds exclusively on the Fabaceae, whereas the currant-lettuce aphid Nasonovia ribisnigri alternates hosts between the Grossulariaceae and Asteraceae. Both species use alarm pheromones to warn of dangers. For N. ribisnigri this pheromone is a single component (E)-beta-farnesene but M. viciae uses a mixture of (E)-beta-farnesene, (-)-alpha-pinene, beta-pinene, and limonene. Odorant-binding proteins (OBP) are believed to capture and transport such semiochemicals to their receptors. Here, we report the first aphid OBP crystal structures and examine their molecular interactions with the alarm pheromone components. Our study reveals some unique structural features: 1) the lack of an internal ligand binding site; 2) a striking groove in the surface of the proteins as a putative binding site; 3) the N-terminus rather than the C-terminus occupies the site closing off the conventional OBP pocket. The results from fluorescent binding assays, molecular docking and dynamics demonstrate that OBP3 from M. viciae can bind to all four alarm pheromone components and the differential ligand binding between these very similar OBP3s from the two aphid species is determined mainly by the direct pi-pi interactions between ligands and the aromatic residues of OBP3s in the binding pocket. | |||
Crystal Structures and Binding Dynamics of Odorant-Binding Protein 3 from two aphid species Megoura viciae and Nasonovia ribisnigri.,Northey T, Venthur H, De Biasio F, Chauviac FX, Cole A, Ribeiro KA Junior, Grossi G, Falabella P, Field LM, Keep NH, Zhou JJ Sci Rep. 2016 Apr 22;6:24739. doi: 10.1038/srep24739. PMID:27102935<ref>PMID:27102935</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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<div class="pdbe-citations 4z45" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
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</StructureSection> | </StructureSection> | ||
Revision as of 10:57, 27 July 2016
Structure of OBP3 from the currant-lettuce aphid Nasonovia ribisnigriStructure of OBP3 from the currant-lettuce aphid Nasonovia ribisnigri
Structural highlights
Publication Abstract from PubMedAphids use chemical cues to locate hosts and find mates. The vetch aphid Megoura viciae feeds exclusively on the Fabaceae, whereas the currant-lettuce aphid Nasonovia ribisnigri alternates hosts between the Grossulariaceae and Asteraceae. Both species use alarm pheromones to warn of dangers. For N. ribisnigri this pheromone is a single component (E)-beta-farnesene but M. viciae uses a mixture of (E)-beta-farnesene, (-)-alpha-pinene, beta-pinene, and limonene. Odorant-binding proteins (OBP) are believed to capture and transport such semiochemicals to their receptors. Here, we report the first aphid OBP crystal structures and examine their molecular interactions with the alarm pheromone components. Our study reveals some unique structural features: 1) the lack of an internal ligand binding site; 2) a striking groove in the surface of the proteins as a putative binding site; 3) the N-terminus rather than the C-terminus occupies the site closing off the conventional OBP pocket. The results from fluorescent binding assays, molecular docking and dynamics demonstrate that OBP3 from M. viciae can bind to all four alarm pheromone components and the differential ligand binding between these very similar OBP3s from the two aphid species is determined mainly by the direct pi-pi interactions between ligands and the aromatic residues of OBP3s in the binding pocket. Crystal Structures and Binding Dynamics of Odorant-Binding Protein 3 from two aphid species Megoura viciae and Nasonovia ribisnigri.,Northey T, Venthur H, De Biasio F, Chauviac FX, Cole A, Ribeiro KA Junior, Grossi G, Falabella P, Field LM, Keep NH, Zhou JJ Sci Rep. 2016 Apr 22;6:24739. doi: 10.1038/srep24739. PMID:27102935[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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