1jw0: Difference between revisions
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|PDB= 1jw0 |SIZE=350|CAPTION= <scene name='initialview01'>1jw0</scene>, resolution 2.5Å | |PDB= 1jw0 |SIZE=350|CAPTION= <scene name='initialview01'>1jw0</scene>, resolution 2.5Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=GUA:GLUTARIC ACID'>GUA</scene> | |LIGAND= <scene name='pdbligand=GUA:GLUTARIC+ACID'>GUA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1jvz|1JVZ]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jw0 OCA], [http://www.ebi.ac.uk/pdbsum/1jw0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jw0 RCSB]</span> | |||
}} | }} | ||
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[[Category: Hol, W G.J.]] | [[Category: Hol, W G.J.]] | ||
[[Category: Kim, Y.]] | [[Category: Kim, Y.]] | ||
[[Category: cephalosporin acylase]] | [[Category: cephalosporin acylase]] | ||
[[Category: glutarate]] | [[Category: glutarate]] | ||
[[Category: glutaryll-7-aca]] | [[Category: glutaryll-7-aca]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:39:50 2008'' | ||
Revision as of 21:39, 30 March 2008
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| , resolution 2.5Å | |||||||
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| Ligands: | , | ||||||
| Related: | 1JVZ
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of cephalosporin acylase in complex with glutarate
OverviewOverview
BACKGROUND: Semisynthetic cephalosporins are primarily synthesized from 7-aminocephalosporanic acid (7-ACA), which is obtained by environmentally toxic chemical deacylation of cephalosporin C (CPC). Thus, the enzymatic conversion of CPC to 7-ACA by cephalosporin acylase (CA) would be of great interest. However, CAs use glutaryl-7-ACA (GL-7-ACA) as a primary substrate and the enzyme has low turnover rates for CPC. RESULTS: The binary complex structures of CA with GL-7-ACA and glutarate (the side-chain of GL-7-ACA) show extensive interactions between the glutaryl moiety of GL-7-ACA and the seven residues that form the side-chain pocket. These interactions explain why the D-alpha-aminoadipyl side-chain of CPC yields a poorer substrate than GL-7-ACA. CONCLUSIONS: This understanding of the nature of substrate specificity may be useful in the design of an enzyme with an improved performance for the conversion of CPC to 7-ACA. Additionally, the catalytic mechanism of the deacylation reaction was revealed by the ligand bound structures.
About this StructureAbout this Structure
1JW0 is a Protein complex structure of sequences from Brevundimonas diminuta. Full crystallographic information is available from OCA.
ReferenceReference
Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity., Kim Y, Hol WG, Chem Biol. 2001 Dec;8(12):1253-64. PMID:11755403
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