5eko: Difference between revisions

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'''Unreleased structure'''


The entry 5eko is ON HOLD  until Paper Publication
==Crystal structure of MAPK13 complex with inhibitor==
 
<StructureSection load='5eko' size='340' side='right' caption='[[5eko]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
Authors: Brett, T.J., Miller, C.A., Yurtsever, Z.
== Structural highlights ==
 
<table><tr><td colspan='2'>[[5eko]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EKO FirstGlance]. <br>
Description: Crystal structure of MAPK13 complex with inhibitor
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=N17:3-(4-METHYL-1H-IMIDAZOL-1-YL)-N-[4-(PYRIDIN-4-YLOXY)PHENYL]BENZAMIDE'>N17</scene></td></tr>
[[Category: Unreleased Structures]]
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4yno|4yno]], [[4myg|4myg]], [[4eyj|4eyj]], [[4eym|4eym]], [[5ekn|5ekn]]</td></tr>
[[Category: Miller, C.A]]
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mitogen-activated_protein_kinase Mitogen-activated protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.24 2.7.11.24] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eko OCA], [http://pdbe.org/5eko PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eko RCSB], [http://www.ebi.ac.uk/pdbsum/5eko PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eko ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/MK13_HUMAN MK13_HUMAN]] Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells.<ref>PMID:9731215</ref> <ref>PMID:11500363</ref> <ref>PMID:11943212</ref> <ref>PMID:15632108</ref> <ref>PMID:17256148</ref> <ref>PMID:18006338</ref> <ref>PMID:18367666</ref> <ref>PMID:20478268</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Mitogen-activated protein kinase]]
[[Category: Brett, T J]]
[[Category: Miller, C A]]
[[Category: Yurtsever, Z]]
[[Category: Yurtsever, Z]]
[[Category: Brett, T.J]]
[[Category: Kinase]]
[[Category: Transferase-transferase inhibitor complex]]

Revision as of 20:58, 13 July 2016

Crystal structure of MAPK13 complex with inhibitorCrystal structure of MAPK13 complex with inhibitor

Structural highlights

5eko is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Activity:Mitogen-activated protein kinase, with EC number 2.7.11.24
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MK13_HUMAN] Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells.[1] [2] [3] [4] [5] [6] [7] [8]

References

  1. ↑ Parker CG, Hunt J, Diener K, McGinley M, Soriano B, Keesler GA, Bray J, Yao Z, Wang XS, Kohno T, Lichenstein HS. Identification of stathmin as a novel substrate for p38 delta. Biochem Biophys Res Commun. 1998 Aug 28;249(3):791-6. PMID:9731215 doi:http://dx.doi.org/10.1006/bbrc.1998.9250
  2. ↑ Knebel A, Morrice N, Cohen P. A novel method to identify protein kinase substrates: eEF2 kinase is phosphorylated and inhibited by SAPK4/p38delta. EMBO J. 2001 Aug 15;20(16):4360-9. PMID:11500363 doi:http://dx.doi.org/10.1093/emboj/20.16.4360
  3. ↑ Buee-Scherrer V, Goedert M. Phosphorylation of microtubule-associated protein tau by stress-activated protein kinases in intact cells. FEBS Lett. 2002 Mar 27;515(1-3):151-4. PMID:11943212
  4. ↑ Feijoo C, Campbell DG, Jakes R, Goedert M, Cuenda A. Evidence that phosphorylation of the microtubule-associated protein Tau by SAPK4/p38delta at Thr50 promotes microtubule assembly. J Cell Sci. 2005 Jan 15;118(Pt 2):397-408. Epub 2005 Jan 4. PMID:15632108 doi:http://dx.doi.org/10.1242/jcs.01655
  5. ↑ Kraft CA, Efimova T, Eckert RL. Activation of PKCdelta and p38delta MAPK during okadaic acid dependent keratinocyte apoptosis. Arch Dermatol Res. 2007 May;299(2):71-83. Epub 2007 Jan 26. PMID:17256148 doi:http://dx.doi.org/10.1007/s00403-006-0727-4
  6. ↑ Pani E, Ferrari S. p38MAPK delta controls c-Myb degradation in response to stress. Blood Cells Mol Dis. 2008 May-Jun;40(3):388-94. Epub 2007 Nov 19. PMID:18006338 doi:http://dx.doi.org/10.1016/j.bcmd.2007.09.010
  7. ↑ Zhou X, Ferraris JD, Dmitrieva NI, Liu Y, Burg MB. MKP-1 inhibits high NaCl-induced activation of p38 but does not inhibit the activation of TonEBP/OREBP: opposite roles of p38alpha and p38delta. Proc Natl Acad Sci U S A. 2008 Apr 8;105(14):5620-5. doi:, 10.1073/pnas.0801453105. Epub 2008 Mar 26. PMID:18367666 doi:http://dx.doi.org/10.1073/pnas.0801453105
  8. ↑ Ozawa S, Ito S, Kato Y, Kubota E, Hata R. Human p38 delta MAP kinase mediates UV irradiation induced up-regulation of the gene expression of chemokine BRAK/CXCL14. Biochem Biophys Res Commun. 2010 Jun 11;396(4):1060-4. doi:, 10.1016/j.bbrc.2010.05.072. Epub 2010 May 15. PMID:20478268 doi:http://dx.doi.org/10.1016/j.bbrc.2010.05.072

5eko, resolution 2.00Å

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