2n8y: Difference between revisions

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-'''Unreleased structure'''
-The entry 2n8y is ON HOLD  until Paper Publication
+==Holo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1==
+<StructureSection load='2n8y' size='340' side='right' caption='[[2n8y]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2n8y]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N8Y FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2n8z|2n8z]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8y OCA], [http://pdbe.org/2n8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n8y RCSB], [http://www.ebi.ac.uk/pdbsum/2n8y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8y ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/ACTN1_HUMAN ACTN1_HUMAN]] F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The activity of several cytosolic proteins critically depends on the concentration of calcium ions. One important intracellular calcium-sensing protein is alpha-actinin-1, the major actin crosslinking protein in focal adhesions and stress fibers. The actin crosslinking activity of alpha-actinin-1 has been proposed to be negatively regulated by calcium, but the underlying molecular mechanisms are poorly understood. To address this, we determined the first high-resolution NMR structure of its functional calmodulin-like domain (CaMD) in calcium-bound and calcium-free form. These structures reveal that in the absence of calcium, CaMD displays a conformationally flexible ensemble that undergoes a structural change upon calcium binding, leading to limited rotation of the N- and C-terminal lobes around the connecting linker and consequent stabilization of the calcium-loaded structure. Mutagenesis experiments, coupled with mass-spectrometry and isothermal calorimetry data designed to validate the calcium binding stoichiometry and binding site, showed that human non-muscle alpha-actinin-1 binds a single calcium ion within the N-terminal lobe. Finally, based on our structural data and analogy with other alpha-actinins, we provide a structural model of regulation of the actin crosslinking activity of alpha-actinin-1 where calcium induced structural stabilisation causes fastening of the juxtaposed actin binding domain, leading to impaired capacity to crosslink actin.
-Authors: Drmota Prebil, S., Slapsak, U., de Almeida Ribeiro, E., Pavsic, M., Ilc, G., Zielinska, K., Hartl, M., Backman, L., Plavec, J., Lenarcic, B., Djinovic-Carugo, K.
+Structure and calcium-binding studies of calmodulin-like domain of human non-muscle alpha-actinin-1.,Drmota Prebil S, Slapsak U, Pavsic M, Ilc G, Puz V, de Almeida Ribeiro E, Anrather D, Hartl M, Backman L, Plavec J, Lenarcic B, Djinovic-Carugo K Sci Rep. 2016 Jun 7;6:27383. doi: 10.1038/srep27383. PMID:27272015<ref>PMID:27272015</ref>
-Description: Holo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 2n8y" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Backman, L]]
 [[Category: Djinovic-Carugo, K]]
-[[Category: Slapsak, U]]
+[[Category: Hartl, M]]
 [[Category: Ilc, G]]
+[[Category: Lenarcic, B]]
+[[Category: Pavsic, M]]
 [[Category: Plavec, J]]
+[[Category: Prebil, S Drmota]]
+[[Category: Ribeiro, E de Almeida]]
+[[Category: Slapsak, U]]
 [[Category: Zielinska, K]]
-[[Category: Hartl, M]]
+[[Category: Calcium binding protein]]
-[[Category: Lenarcic, B]]
-[[Category: Drmota Prebil, S]]
-[[Category: Pavsic, M]]
-[[Category: De Almeida Ribeiro, E]]