1j3r: Difference between revisions
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|PDB= 1j3r |SIZE=350|CAPTION= <scene name='initialview01'>1j3r</scene>, resolution 2.18Å | |PDB= 1j3r |SIZE=350|CAPTION= <scene name='initialview01'>1j3r</scene>, resolution 2.18Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=6PG:6-PHOSPHOGLUCONIC+ACID'>6PG</scene> | |LIGAND= <scene name='pdbligand=6PG:6-PHOSPHOGLUCONIC+ACID'>6PG</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1j3p|1J3P]], [[1j3q|1J3Q]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j3r OCA], [http://www.ebi.ac.uk/pdbsum/1j3r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1j3r RCSB]</span> | |||
}} | }} | ||
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[[Category: Shoun, H.]] | [[Category: Shoun, H.]] | ||
[[Category: Wakagi, T.]] | [[Category: Wakagi, T.]] | ||
[[Category: archaea]] | [[Category: archaea]] | ||
[[Category: cupin superfamily]] | [[Category: cupin superfamily]] | ||
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[[Category: phosphoglucose isomerase]] | [[Category: phosphoglucose isomerase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:28:28 2008'' | ||
Revision as of 21:28, 30 March 2008
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| , resolution 2.18Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Glucose-6-phosphate isomerase, with EC number 5.3.1.9 | ||||||
| Related: | 1J3P, 1J3Q
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Thermococcus litoralis phosphogrucose isomerase complexed with gluconate-6-phosphate
OverviewOverview
The gene encoding phosphoglucose isomerase was cloned from Thermococcus litoralis, and functionally expressed in Escherichia coli. The purified enzyme, a homodimer of 21.5 kDa subunits, was biochemically characterized. The inhibition constants for four competitive inhibitors were determined. The enzyme contained 1.25 mol Fe and 0.24 mol Zn per dimer. The activity was enhanced by the addition of Fe(2+), but inhibited by Zn(2+) and EDTA. Enzymes with mutations in conserved histidine and glutamate residues in their cupin motifs contained no metals, and showed large decreases in k(cat). The circular dichroism spectra of the mutant enzymes and the wild type enzyme were essentially the same but with slight differences.
About this StructureAbout this Structure
1J3R is a Single protein structure of sequence from Thermococcus litoralis. Full crystallographic information is available from OCA.
ReferenceReference
Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis., Jeong JJ, Fushinobu S, Ito S, Jeon BS, Shoun H, Wakagi T, FEBS Lett. 2003 Jan 30;535(1-3):200-4. PMID:12560104
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