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==Crystal structure of a T1-like thiolase (CoA-complex) from Mycobacterium smegmatis== | |||
<StructureSection load='5bz4' size='340' side='right' caption='[[5bz4]], [[Resolution|resolution]] 2.43Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5bz4]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BZ4 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5byv|5byv]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bz4 OCA], [http://pdbe.org/5bz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bz4 RCSB], [http://www.ebi.ac.uk/pdbsum/5bz4 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Thiolases catalyze the degradation and synthesis of 3-ketoacyl-CoA molecules. Here, the crystal structures of a T1-like thiolase (MSM-13 thiolase) from Mycobacterium smegmatis in apo and liganded forms are described. Systematic comparisons of six crystallographically independent unliganded MSM-13 thiolase tetramers (dimers of tight dimers) from three different crystal forms revealed that the two tight dimers are connected to a rigid tetramerization domain via flexible hinge regions, generating an asymmetric tetramer. In the liganded structure, CoA is bound to those subunits that are rotated towards the tip of the tetramerization loop of the opposing dimer, suggesting that this loop is important for substrate binding. The hinge regions responsible for this rotation occur near Val123 and Arg149. The Lalpha1-covering loop-Lalpha2 region, together with the Nbeta2-Nalpha2 loop of the adjacent subunit, defines a specificity pocket that is larger and more polar than those of other tetrameric thiolases, suggesting that MSM-13 thiolase has a distinct substrate specificity. Consistent with this finding, only residual activity was detected with acetoacetyl-CoA as the substrate in the degradative direction. No activity was observed with acetyl-CoA in the synthetic direction. Structural comparisons with other well characterized thiolases suggest that MSM-13 thiolase is probably a degradative thiolase that is specific for 3-ketoacyl-CoA molecules with polar, bulky acyl chains. | |||
Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis.,Janardan N, Harijan RK, Kiema TR, Wierenga RK, Murthy MR Acta Crystallogr D Biol Crystallogr. 2015 Dec 1;71(Pt 12):2479-93. doi:, 10.1107/S1399004715019331. Epub 2015 Nov 27. PMID:26627655<ref>PMID:26627655</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5bz4" style="background-color:#fffaf0;"></div> | |||
== References == | |||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Harijan, R K]] | |||
[[Category: Janardan, N]] | [[Category: Janardan, N]] | ||
[[Category: Kiema, T R]] | |||
[[Category: Murthy, M R.N]] | |||
[[Category: Wierenga, R K]] | |||
[[Category: Coa-complex]] | |||
[[Category: T1]] | |||
[[Category: Tetramer]] | |||
[[Category: Thiolase]] | |||
[[Category: Transferase]] | |||
Revision as of 18:49, 1 June 2016
Crystal structure of a T1-like thiolase (CoA-complex) from Mycobacterium smegmatisCrystal structure of a T1-like thiolase (CoA-complex) from Mycobacterium smegmatis
Structural highlights
Publication Abstract from PubMedThiolases catalyze the degradation and synthesis of 3-ketoacyl-CoA molecules. Here, the crystal structures of a T1-like thiolase (MSM-13 thiolase) from Mycobacterium smegmatis in apo and liganded forms are described. Systematic comparisons of six crystallographically independent unliganded MSM-13 thiolase tetramers (dimers of tight dimers) from three different crystal forms revealed that the two tight dimers are connected to a rigid tetramerization domain via flexible hinge regions, generating an asymmetric tetramer. In the liganded structure, CoA is bound to those subunits that are rotated towards the tip of the tetramerization loop of the opposing dimer, suggesting that this loop is important for substrate binding. The hinge regions responsible for this rotation occur near Val123 and Arg149. The Lalpha1-covering loop-Lalpha2 region, together with the Nbeta2-Nalpha2 loop of the adjacent subunit, defines a specificity pocket that is larger and more polar than those of other tetrameric thiolases, suggesting that MSM-13 thiolase has a distinct substrate specificity. Consistent with this finding, only residual activity was detected with acetoacetyl-CoA as the substrate in the degradative direction. No activity was observed with acetyl-CoA in the synthetic direction. Structural comparisons with other well characterized thiolases suggest that MSM-13 thiolase is probably a degradative thiolase that is specific for 3-ketoacyl-CoA molecules with polar, bulky acyl chains. Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis.,Janardan N, Harijan RK, Kiema TR, Wierenga RK, Murthy MR Acta Crystallogr D Biol Crystallogr. 2015 Dec 1;71(Pt 12):2479-93. doi:, 10.1107/S1399004715019331. Epub 2015 Nov 27. PMID:26627655[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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