1iv4: Difference between revisions

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Revision as of 21:25, 30 March 2008

File:1iv4.gif

, resolution 1.55Å

Ligands:

,

Activity:

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, with EC number 4.6.1.12

Related:

1IV1, 1IV2, 1IV3

Resources:

FirstGlance, OCA, PDBsum, RCSB

Coordinates:

save as pdb, mmCIF, xml

Structure of 2C-Methyl-D-erythritol-2,4-cyclodiphosphate Synthase (bound form Substrate)

OverviewOverview

Precursors for isoprenoid synthesis are essential in all organisms. These compounds are synthesized by one of two known routes: the well characterized mevalonate pathway or a recently discovered non-mevalonate route which is used in many bacteria and human pathogens. Since the second pathway is both vital and unlike any found in humans, enzymes catalysing reactions along this synthetic route are possible drug targets. The structure of one such enzyme from the thermophilic bacterium Thermus thermophilus has been solved to high resolution in the presence of substrate and with a substrate analogue. Enzyme co-crystallized with substrate shows only one product, cytosine monophosphate (CMP), in the active site. At the high resolution of the refinement (1.6 A) the positions and coordination of the magnesium ions in the active site are clearly seen.

About this StructureAbout this Structure

1IV4 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Structure and catalytic mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) synthase, an enzyme in the non-mevalonate pathway of isoprenoid synthesis., Kishida H, Wada T, Unzai S, Kuzuyama T, Takagi M, Terada T, Shirouzu M, Yokoyama S, Tame JR, Park SY, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):23-31. Epub 2002, Dec 19. PMID:12499535

Page seeded by OCA on Sun Mar 30 21:25:03 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 4: / Line 4: @@
 |PDB= 1iv4 |SIZE=350|CAPTION= <scene name='initialview01'>1iv4</scene>, resolution 1.55&Aring;
 |SITE=
-|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=C5P:CYTIDINE-5&#39;-MONOPHOSPHATE'>C5P</scene>
+|LIGAND= <scene name='pdbligand=C5P:CYTIDINE-5&#39;-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
-|ACTIVITY= [http://en.wikipedia.org/wiki/2-C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.12 4.6.1.12]
+|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/2-C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.12 4.6.1.12] </span>
 |GENE=
+|DOMAIN=
+|RELATEDENTRY=[[1iv1|1IV1]], [[1iv2|1IV2]], [[1iv3|1IV3]]
+|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1iv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iv4 OCA], [http://www.ebi.ac.uk/pdbsum/1iv4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1iv4 RCSB]</span>
 }}
@@ Line 33: / Line 36: @@
 [[Category: Wada, T.]]
 [[Category: Yokoyama, S.]]
-[[Category: C5P]]
-[[Category: MG]]
 [[Category: isoprenoid]]
 [[Category: non-mevalonate]]
@@ Line 42: / Line 43: @@
 [[Category: synthase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 12:15:55 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:25:03 2008''