1ire: Difference between revisions
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|PDB= 1ire |SIZE=350|CAPTION= <scene name='initialview01'>1ire</scene>, resolution 1.80Å | |PDB= 1ire |SIZE=350|CAPTION= <scene name='initialview01'>1ire</scene>, resolution 1.80Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CO:COBALT (II) ION'>CO</scene> | |LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ire FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ire OCA], [http://www.ebi.ac.uk/pdbsum/1ire PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ire RCSB]</span> | |||
}} | }} | ||
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[[Category: Miyanaga, A.]] | [[Category: Miyanaga, A.]] | ||
[[Category: Wakagi, T.]] | [[Category: Wakagi, T.]] | ||
[[Category: cysteine-sulfenic acid]] | [[Category: cysteine-sulfenic acid]] | ||
[[Category: cysteine-sulfinic acid]] | [[Category: cysteine-sulfinic acid]] | ||
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[[Category: post-translational modification]] | [[Category: post-translational modification]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:23:35 2008'' | ||
Revision as of 21:23, 30 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Activity: | Nitrile hydratase, with EC number 4.2.1.84 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Co-type nitrile hydratase from Pseudonocardia thermophila
OverviewOverview
The crystal structure of cobalt-containing nitrile hydratase from Pseudonocardia thermophila JCM 3095 at 1.8 A resolution revealed the structure of the noncorrin cobalt at the catalytic center. Two cysteine residues (alphaCys(111) and alphaCys(113)) coordinated to the cobalt were posttranslationally modified to cysteine-sulfinic acid and to cysteine-sulfenic acid, respectively, like in iron-containing nitrile hydratase. A tryptophan residue (betaTrp(72)), which may be involved in substrate binding, replaced the tyrosine residue of iron-containing nitrile hydratase. The difference seems to be responsible for the preference for aromatic nitriles rather than aliphatic ones of cobalt-containing nitrile hydratase.
About this StructureAbout this Structure
1IRE is a Protein complex structure of sequences from Pseudonocardia thermophila. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of cobalt-containing nitrile hydratase., Miyanaga A, Fushinobu S, Ito K, Wakagi T, Biochem Biophys Res Commun. 2001 Nov 16;288(5):1169-74. PMID:11700034
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