1io7: Difference between revisions
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|PDB= 1io7 |SIZE=350|CAPTION= <scene name='initialview01'>1io7</scene>, resolution 1.5Å | |PDB= 1io7 |SIZE=350|CAPTION= <scene name='initialview01'>1io7</scene>, resolution 1.5Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene> | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1io8|1IO8]], [[1io9|1IO9]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1io7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1io7 OCA], [http://www.ebi.ac.uk/pdbsum/1io7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1io7 RCSB]</span> | |||
}} | }} | ||
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[[Category: Sligar, S G.]] | [[Category: Sligar, S G.]] | ||
[[Category: Yamane, K.]] | [[Category: Yamane, K.]] | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
[[Category: cytochromo p450]] | [[Category: cytochromo p450]] | ||
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[[Category: thermophilic]] | [[Category: thermophilic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:22:22 2008'' | ||
Revision as of 21:22, 30 March 2008
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| , resolution 1.5Å | |||||||
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| Ligands: | |||||||
| Related: | 1IO8, 1IO9
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THERMOPHILIC CYTOCHROME P450 (CYP119) FROM SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION STRUCTURAL ORIGIN OF ITS THERMOSTABILITY AND FUNCTIONAL PROPERTIES
OverviewOverview
Crystal structures of a thermostable cytochrome P450 (CYP119) and a site-directed mutant, (Phe24Leu), from the acidothermophilic archaea Sulfolobus solfataricus were determined at 1.5-2.0 A resolution. We identify important crystallographic waters in the ferric heme pocket, observe protein conformational changes upon inhibitor binding, and detect a unique distribution of surface charge not found in other P450s. An analysis of factors contributing to thermostability of CYP119 of these high resolution structures shows an apparent increase in clustering of aromatic residues and optimum stacking. The contribution of aromatic stacking was investigated further with the mutant crystal structure and differential scanning calorimetry.
About this StructureAbout this Structure
1IO7 is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
ReferenceReference
Thermophilic cytochrome P450 (CYP119) from Sulfolobus solfataricus: high resolution structure and functional properties., Park SY, Yamane K, Adachi S, Shiro Y, Weiss KE, Maves SA, Sligar SG, J Inorg Biochem. 2002 Sep 20;91(4):491-501. PMID:12237217
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