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==Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 2.5 mM Na+ and 1mM Ca2+== | |||
<StructureSection load='5jdf' size='340' side='right' caption='[[5jdf]], [[Resolution|resolution]] 2.65Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5jdf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JDF FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MYS:PENTADECANE'>MYS</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5jdq|5jdq]], [[5jdn|5jdn]], [[5jdm|5jdm]], [[5jdl|5jdl]], [[5jdh|5jdh]], [[5jdg|5jdg]], [[5hwx|5hwx]], [[5hwy|5hwy]], [[5hxc|5hxc]], [[5hxe|5hxe]], [[5hxh|5hxh]], [[5hxr|5hxr]], [[5hxs|5hxs]], [[5hya|5hya]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jdf OCA], [http://pdbe.org/5jdf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jdf RCSB], [http://www.ebi.ac.uk/pdbsum/5jdf PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Sodium/calcium (Na(+)/Ca(2+)) exchangers (NCX) are membrane transporters that play an essential role in maintaining the homeostasis of cytosolic Ca(2+) for cell signaling. We demonstrated the Na(+)/Ca(2+)-exchange function of an NCX from Methanococcus jannaschii (NCX_Mj) and report its 1.9 angstrom crystal structure in an outward-facing conformation. Containing 10 transmembrane helices, the two halves of NCX_Mj share a similar structure with opposite orientation. Four ion-binding sites cluster at the center of the protein: one specific for Ca(2+) and three that likely bind Na(+). Two passageways allow for Na(+) and Ca(2+) access to the central ion-binding sites from the extracellular side. Based on the symmetry of NCX_Mj and its ability to catalyze bidirectional ion-exchange reactions, we propose a structure model for the inward-facing NCX_Mj. | |||
Structural insight into the ion-exchange mechanism of the sodium/calcium exchanger.,Liao J, Li H, Zeng W, Sauer DB, Belmares R, Jiang Y Science. 2012 Feb 10;335(6069):686-90. PMID:22323814<ref>PMID:22323814</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5jdf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Faraldo-Gomez, J D]] | |||
[[Category: Jiang, Y X]] | |||
[[Category: Liao, J]] | [[Category: Liao, J]] | ||
[[Category: | [[Category: Calcium signalling]] | ||
[[Category: | [[Category: Induced conformational change]] | ||
[[Category: Membrane protein]] | |||
[[Category: Membrane transporter]] | |||
[[Category: Na+/ca2+ exchange]] | |||
Revision as of 19:56, 15 May 2016
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 2.5 mM Na+ and 1mM Ca2+Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 2.5 mM Na+ and 1mM Ca2+
Structural highlights
Publication Abstract from PubMedSodium/calcium (Na(+)/Ca(2+)) exchangers (NCX) are membrane transporters that play an essential role in maintaining the homeostasis of cytosolic Ca(2+) for cell signaling. We demonstrated the Na(+)/Ca(2+)-exchange function of an NCX from Methanococcus jannaschii (NCX_Mj) and report its 1.9 angstrom crystal structure in an outward-facing conformation. Containing 10 transmembrane helices, the two halves of NCX_Mj share a similar structure with opposite orientation. Four ion-binding sites cluster at the center of the protein: one specific for Ca(2+) and three that likely bind Na(+). Two passageways allow for Na(+) and Ca(2+) access to the central ion-binding sites from the extracellular side. Based on the symmetry of NCX_Mj and its ability to catalyze bidirectional ion-exchange reactions, we propose a structure model for the inward-facing NCX_Mj. Structural insight into the ion-exchange mechanism of the sodium/calcium exchanger.,Liao J, Li H, Zeng W, Sauer DB, Belmares R, Jiang Y Science. 2012 Feb 10;335(6069):686-90. PMID:22323814[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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