1i9y: Difference between revisions
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1i9z|1I9Z]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i9y OCA], [http://www.ebi.ac.uk/pdbsum/1i9y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i9y RCSB]</span> | |||
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[[Category: Tsujishita, Y.]] | [[Category: Tsujishita, Y.]] | ||
[[Category: York, J D.]] | [[Category: York, J D.]] | ||
[[Category: inositol 5-phosphatase]] | [[Category: inositol 5-phosphatase,]] | ||
[[Category: ipp5c]] | [[Category: ipp5c]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:16:47 2008'' | ||
Revision as of 21:16, 30 March 2008
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| , resolution 2.0Å | |||||||
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| Related: | 1I9Z
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 5-PHOSPHATASE DOMAIN (IPP5C) OF SPSYNAPTOJANIN
OverviewOverview
Inositol polyphosphate 5-phosphatases are central to intracellular processes ranging from membrane trafficking to Ca(2+) signaling, and defects in this activity result in the human disease Lowe syndrome. The 1.8 resolution structure of the inositol polyphosphate 5-phosphatase domain of SPsynaptojanin bound to Ca(2+) and inositol (1,4)-bisphosphate reveals a fold and an active site His and Asp pair resembling those of several Mg(2+)-dependent nucleases. Additional loops mediate specific inositol polyphosphate contacts. The 4-phosphate of inositol (1,4)-bisphosphate is misoriented by 4.6 compared to the reactive geometry observed in the apurinic/apyrimidinic endonuclease 1, explaining the dephosphorylation site selectivity of the 5-phosphatases. Based on the structure, a series of mutants are described that exhibit altered substrate specificity providing general determinants for substrate recognition.
About this StructureAbout this Structure
1I9Y is a Single protein structure of sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.
ReferenceReference
Specificity determinants in phosphoinositide dephosphorylation: crystal structure of an archetypal inositol polyphosphate 5-phosphatase., Tsujishita Y, Guo S, Stolz LE, York JD, Hurley JH, Cell. 2001 May 4;105(3):379-89. PMID:11348594
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