5i6c: Difference between revisions
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The | ==The structure of the eukaryotic purine/H+ symporter, UapA, in complex with Xanthine== | ||
<StructureSection load='5i6c' size='340' side='right' caption='[[5i6c]], [[Resolution|resolution]] 3.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5i6c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I6C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I6C FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=XAN:XANTHINE'>XAN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i6c OCA], [http://pdbe.org/5i6c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i6c RCSB], [http://www.ebi.ac.uk/pdbsum/5i6c PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/UAPA_EMENI UAPA_EMENI]] Uric acid-xanthine transporter.<ref>PMID:15953615</ref> <ref>PMID:16096268</ref> <ref>PMID:20002879</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The uric acid/xanthine H(+) symporter, UapA, is a high-affinity purine transporter from the filamentous fungus Aspergillus nidulans. Here we present the crystal structure of a genetically stabilized version of UapA (UapA-G411VDelta1-11) in complex with xanthine. UapA is formed from two domains, a core domain and a gate domain, similar to the previously solved uracil transporter UraA, which belongs to the same family. The structure shows UapA in an inward-facing conformation with xanthine bound to residues in the core domain. Unlike UraA, which was observed to be a monomer, UapA forms a dimer in the crystals with dimer interactions formed exclusively through the gate domain. Analysis of dominant negative mutants is consistent with dimerization playing a key role in transport. We postulate that UapA uses an elevator transport mechanism likely to be shared with other structurally homologous transporters including anion exchangers and prestin. | |||
Structure of eukaryotic purine/H(+) symporter UapA suggests a role for homodimerization in transport activity.,Alguel Y, Amillis S, Leung J, Lambrinidis G, Capaldi S, Scull NJ, Craven G, Iwata S, Armstrong A, Mikros E, Diallinas G, Cameron AD, Byrne B Nat Commun. 2016 Apr 18;7:11336. doi: 10.1038/ncomms11336. PMID:27088252<ref>PMID:27088252</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5i6c" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Alguel, Y]] | [[Category: Alguel, Y]] | ||
[[Category: Amillis, S]] | [[Category: Amillis, S]] | ||
[[Category: | [[Category: Armstrong, A]] | ||
[[Category: Byrne, B]] | [[Category: Byrne, B]] | ||
[[Category: Cameron, A | [[Category: Cameron, A D]] | ||
[[Category: Capaldi, S]] | |||
[[Category: Craven, G]] | [[Category: Craven, G]] | ||
[[Category: Diallinas, G]] | [[Category: Diallinas, G]] | ||
[[Category: | [[Category: Iwata, S]] | ||
[[Category: Lambrinidis, G]] | [[Category: Lambrinidis, G]] | ||
[[Category: Leung, J]] | |||
[[Category: Mikros, E]] | |||
[[Category: Scull, N J]] | |||
[[Category: Membrane protein eukaryotic uric acid/xanthine h+ symporter]] | |||
[[Category: Transport protein]] | |||