1hf3: Difference between revisions
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|PDB= 1hf3 |SIZE=350|CAPTION= <scene name='initialview01'>1hf3</scene>, resolution 1.95Å | |PDB= 1hf3 |SIZE=350|CAPTION= <scene name='initialview01'>1hf3</scene>, resolution 1.95Å | ||
|SITE= <scene name='pdbsite=AC2:Cd+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC4:Cd+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=NAA:Nad+Binding+Site+For+Chain+A'>NAA</scene>, <scene name='pdbsite=NAB:Nad+Binding+Site+For+Chain+B'>NAB</scene>, <scene name='pdbsite=OHA:Active+Site+w.+Zn+Bound+Hydroxide'>OHA</scene> and <scene name='pdbsite=OHB:Cd+Binding+Site+For+Chain+B'>OHB</scene> | |SITE= <scene name='pdbsite=AC2:Cd+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC4:Cd+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=NAA:Nad+Binding+Site+For+Chain+A'>NAA</scene>, <scene name='pdbsite=NAB:Nad+Binding+Site+For+Chain+B'>NAB</scene>, <scene name='pdbsite=OHA:Active+Site+w.+Zn+Bound+Hydroxide'>OHA</scene> and <scene name='pdbsite=OHB:Cd+Binding+Site+For+Chain+B'>OHB</scene> | ||
|LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hf3 OCA], [http://www.ebi.ac.uk/pdbsum/1hf3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hf3 RCSB]</span> | |||
}} | }} | ||
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[[Category: Merli, A.]] | [[Category: Merli, A.]] | ||
[[Category: Morris, R J.]] | [[Category: Morris, R J.]] | ||
[[Category: oxidoreductase(nad(a)-choh(d))]] | [[Category: oxidoreductase(nad(a)-choh(d))]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:03:48 2008'' | ||
Revision as of 21:03, 30 March 2008
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| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , and | ||||||
| Ligands: | , , | ||||||
| Activity: | Alcohol dehydrogenase, with EC number 1.1.1.1 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ATOMIC X-RAY STRUCTURE OF LIVER ALCOHOL DEHYDROGENASE CONTAINING CADMIUM AND A HYDROXIDE ADDUCT TO NADH
OverviewOverview
Atomic (1 A) resolution x-ray structures of horse liver alcohol dehydrogenase in complex with NADH revealed the formation of an adduct in the active site between a metal-bound water and NADH. Furthermore, a pronounced distortion of the pyridine ring of NADH was observed. A series of quantum chemical calculations on the water-nicotinamide adduct showed that the puckering of the pyridine ring in the crystal structures can only be reproduced when the water is considered a hydroxide ion. These observations provide fundamental insight into the enzymatic activation of NADH for hydride transfer.
About this StructureAbout this Structure
1HF3 is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.
ReferenceReference
On the enzymatic activation of NADH., Meijers R, Morris RJ, Adolph HW, Merli A, Lamzin VS, Cedergren-Zeppezauer ES, J Biol Chem. 2001 Mar 23;276(12):9316-21. Epub 2000 Dec 28. PMID:11134046
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