5e9u: Difference between revisions

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-'''Unreleased structure'''
-The entry 5e9u is ON HOLD  until Paper Publication
+==Crystal structure of GtfA/B complex bound to UDP and GlcNAc==
+<StructureSection load='5e9u' size='340' side='right' caption='[[5e9u]], [[Resolution|resolution]] 3.84&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5e9u]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E9U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5E9U FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5e9t|5e9t]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5e9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e9u OCA], [http://pdbe.org/5e9u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5e9u RCSB], [http://www.ebi.ac.uk/pdbsum/5e9u PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/GTF1_STRGN GTF1_STRGN]] An N-acetylglucosaminyl transferase that is part of the accessory SecA2/SecY2 system specifically required to export GspB, a serine-rich repeat cell wall protein usually encoded upstream in the same operon. Required for correct glycosylation of GspB. Upon coexpression in E.coli with Gtf2 (GtfB) glycosylates GspB constructs. Glycosylation probably occurs intracellularly.<ref>PMID:15489421</ref>  [[http://www.uniprot.org/uniprot/GTF2_STRGN GTF2_STRGN]] A stabilizing protein that is part of the accessory SecA2/SecY2 system specifically required to export GspB, a serine-rich repeat cell wall protein encoded upstream in the same operon. Required for correct glycosylation of GspB. Upon coexpression in E.coli with Gtf1 (GtfA) glycosylates GspB constructs. Stabilizes the glycosylation activity of Gtf1 (By similarity).<ref>PMID:15489421</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+O-glycosylation of Ser and Thr residues is an important process in all organisms, which is only poorly understood. Such modification is required for the export and function of adhesin proteins that mediate the attachment of pathogenic Gram-positive bacteria to host cells. Here, we have analyzed the mechanism by which the cytosolic O-glycosyltransferase GtfA/B of Streptococcus gordonii modifies the Ser/Thr-rich repeats of adhesin. The enzyme is a tetramer containing two molecules each of GtfA and GtfB. The two subunits have the same fold, but only GtfA contains an active site, whereas GtfB provides the primary binding site for adhesin. During a first phase of glycosylation, the conformation of GtfB is restrained by GtfA to bind substrate with unmodified Ser/Thr residues. In a slow second phase, GtfB recognizes residues that are already modified with N-acetylglucosamine, likely by converting into a relaxed conformation in which one interface with GtfA is broken. These results explain how the glycosyltransferase modifies a progressively changing substrate molecule.
-Authors: Chen, Y., Rapoport, T.A.
+Mechanism of a cytosolic O-glycosyltransferase essential for the synthesis of a bacterial adhesion protein.,Chen Y, Seepersaud R, Bensing BA, Sullam PM, Rapoport TA Proc Natl Acad Sci U S A. 2016 Feb 16. pii: 201600494. PMID:26884191<ref>PMID:26884191</ref>
-Description: Crystal structure of GtfA/B complex bound to UDP and GlcNAc
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 5e9u" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Chen, Y]]
-[[Category: Rapoport, T.A]]
+[[Category: Rapoport, T A]]
+[[Category: Accessory protein translocation]]
+[[Category: Complex]]
+[[Category: Glycosyltransferase]]
+[[Category: Transferase-chaperone complex]]