2bte: Difference between revisions
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
Leucyl-tRNA synthetase (LeuRS) has a specific post-transfer editing, activity directed against mischarged isoleucine and similar noncognate, amino acids. We describe the post-transfer-editing and product complexes, of Thermus thermophilus LeuRS (LeuRSTT) with tRNA(Leu) at 2.9- to 3.3-A, resolution. In the post-transfer-editing configuration, A76 binds in the, editing active site exactly as previously found for the adenosine moiety, of a small-molecule editing-substrate analog. The 60 C-terminal residues, of LeuRSTT, unseen in previous structures, fold into a compact domain, flexibly linked to the rest of the molecule and interacting with the, G19-C56 tertiary base pair of tRNA(Leu). LeuRS recognition of tRNA(Leu), depends essentially on tRNA shape rather than base-specific interactions., . | Leucyl-tRNA synthetase (LeuRS) has a specific post-transfer editing, activity directed against mischarged isoleucine and similar noncognate, amino acids. We describe the post-transfer-editing and product complexes, of Thermus thermophilus LeuRS (LeuRSTT) with tRNA(Leu) at 2.9- to 3.3-A, resolution. In the post-transfer-editing configuration, A76 binds in the, editing active site exactly as previously found for the adenosine moiety, of a small-molecule editing-substrate analog. The 60 C-terminal residues, of LeuRSTT, unseen in previous structures, fold into a compact domain, flexibly linked to the rest of the molecule and interacting with the, G19-C56 tertiary base pair of tRNA(Leu). LeuRS recognition of tRNA(Leu), depends essentially on tRNA shape rather than base-specific interactions., The structures show that considerable domain rotations, notably of the, editing domain, accompany the tRNA-3' end dynamics associated successively, with aminoacylation, post-transfer editing and product release. | ||
==About this Structure== | ==About this Structure== | ||
2BTE is a | 2BTE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with ZN, HG, SO4, NVA and LEU as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BTE OCA]. | ||
==Reference== | ==Reference== | ||
| Line 28: | Line 28: | ||
[[Category: synthetase]] | [[Category: synthetase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:26:56 2007'' | ||