Exoenzyme: Difference between revisions
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'''Exoenzymes''' (Exo) are secreted by cells and break large macromolecules outside the cells. The breakdown components can pass through the cell wall. '''Exo C3''' is a ''Clostridium butulinum'' toxin which binds [[NAD]], splits it into ADP-ribose and nicotinamide and transfers the ADP-ribose to a protein substrate. '''Exo S''' and '''Exo T''' are secreted by ''Pseudomonas aeruginosa'' and has GTPase-activating activity as well as ADP-ribosyltransferase activity<ref>PMID:11114250</ref> . | '''Exoenzymes''' (Exo) are secreted by cells and break large macromolecules outside the cells. The breakdown components can pass through the cell wall. '''Exo C3''' is a ''Clostridium butulinum'' toxin which binds [[NAD]], splits it into ADP-ribose and nicotinamide and transfers the ADP-ribose to a protein substrate. '''Exo S''' and '''Exo T''' are secreted by ''Pseudomonas aeruginosa'' and has GTPase-activating activity as well as ADP-ribosyltransferase activity<ref>PMID:11114250</ref> . | ||
<scene name='59/593306/Cv/3'>NAD binding site</scene> in exoenzyme C3 catalytic domain (PDB code [[2c8c]]).<ref>PMID:18369192</ref> | <scene name='59/593306/Cv/3'>NAD binding site</scene>. | ||
<scene name='59/593306/Cv/5'>ADP binding site</scene> in exoenzyme C3 catalytic domain (PDB code [[2c8c]]). <ref>PMID:18369192</ref> | |||
</StructureSection> | </StructureSection> | ||
Revision as of 15:26, 14 February 2016
Exoenzymes (Exo) are secreted by cells and break large macromolecules outside the cells. The breakdown components can pass through the cell wall. Exo C3 is a Clostridium butulinum toxin which binds NAD, splits it into ADP-ribose and nicotinamide and transfers the ADP-ribose to a protein substrate. Exo S and Exo T are secreted by Pseudomonas aeruginosa and has GTPase-activating activity as well as ADP-ribosyltransferase activity[1] . . in exoenzyme C3 catalytic domain (PDB code 2c8c). [2]
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3D structures of exoenzyme3D structures of exoenzyme
Updated on 14-February-2016
ReferencesReferences
- ↑ Han S, Arvai AS, Clancy SB, Tainer JA. Crystal structure and novel recognition motif of rho ADP-ribosylating C3 exoenzyme from Clostridium botulinum: structural insights for recognition specificity and catalysis. J Mol Biol. 2001 Jan 5;305(1):95-107. PMID:11114250 doi:10.1006/jmbi.2000.4292
- ↑ Menetrey J, Flatau G, Boquet P, Menez A, Stura EA. Structural basis for the NAD-hydrolysis mechanism and the ARTT-loop plasticity of C3 exoenzymes. Protein Sci. 2008 May;17(5):878-86. Epub 2008 Mar 27. PMID:18369192 doi:10.1110/ps.073398508