1g1r: Difference between revisions
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|PDB= 1g1r |SIZE=350|CAPTION= <scene name='initialview01'>1g1r</scene>, resolution 3.4Å | |PDB= 1g1r |SIZE=350|CAPTION= <scene name='initialview01'>1g1r</scene>, resolution 3.4Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAG:ALPHA-METHYL-N-ACETYL-D-GLUCOSAMINE'>MAG</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1g1q|1G1Q]], [[1g1s|1G1S]], [[1g1t|1G1T]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g1r OCA], [http://www.ebi.ac.uk/pdbsum/1g1r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g1r RCSB]</span> | |||
}} | }} | ||
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==Overview== | ==Overview== | ||
P-, E- and L-selectin constitute a family of cell adhesion receptors that mediate the initial tethering and rolling of leukocytes on inflamed endothelium as a prelude to their firm attachment and extravasation into tissues. The selectins bind weakly to sialyl Lewisx (SLe(X))-like glycans, but with high-affinity to specific glycoprotein counterreceptors, including PSGL-1. Here, we report crystal structures of human P- and E-selectin constructs containing the lectin and EGF (LE) domains co-complexed with SLe(X). We also present the crystal structure of P-selectin LE co-complexed with the N-terminal domain of human PSGL-1 modified by both tyrosine sulfation and SLe(X). These structures reveal differences in how E- and P-selectin bind SLe(X) and the molecular basis of the high-affinity interaction between P-selectin and PSGL-1. | P-, E- and L-selectin constitute a family of cell adhesion receptors that mediate the initial tethering and rolling of leukocytes on inflamed endothelium as a prelude to their firm attachment and extravasation into tissues. The selectins bind weakly to sialyl Lewisx (SLe(X))-like glycans, but with high-affinity to specific glycoprotein counterreceptors, including PSGL-1. Here, we report crystal structures of human P- and E-selectin constructs containing the lectin and EGF (LE) domains co-complexed with SLe(X). We also present the crystal structure of P-selectin LE co-complexed with the N-terminal domain of human PSGL-1 modified by both tyrosine sulfation and SLe(X). These structures reveal differences in how E- and P-selectin bind SLe(X) and the molecular basis of the high-affinity interaction between P-selectin and PSGL-1. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Camphausen, R T.]] | [[Category: Camphausen, R T.]] | ||
[[Category: Somers, W S.]] | [[Category: Somers, W S.]] | ||
[[Category: adhesion molecule]] | [[Category: adhesion molecule]] | ||
[[Category: egf]] | [[Category: egf]] | ||
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[[Category: slex]] | [[Category: slex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:34:46 2008'' | ||