1g07: Difference between revisions
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|PDB= 1g07 |SIZE=350|CAPTION= <scene name='initialview01'>1g07</scene>, resolution 1.7Å | |PDB= 1g07 |SIZE=350|CAPTION= <scene name='initialview01'>1g07</scene>, resolution 1.7Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g07 OCA], [http://www.ebi.ac.uk/pdbsum/1g07 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g07 RCSB]</span> | |||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
1G07 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | 1G07 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G07 OCA]. | ||
==Reference== | ==Reference== | ||
Structural and thermodynamic analysis of the binding of solvent at internal sites in T4 lysozyme., Xu J, Baase WA, Quillin ML, Baldwin EP, Matthews BW, Protein Sci. 2001 May;10(5):1067-78. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11316887 11316887] | Structural and thermodynamic analysis of the binding of solvent at internal sites in T4 lysozyme., Xu J, Baase WA, Quillin ML, Baldwin EP, Matthews BW, Protein Sci. 2001 May;10(5):1067-78. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11316887 11316887] | ||
[[Category: | [[Category: Enterobacteria phage t4]] | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Quillin, M L.]] | [[Category: Quillin, M L.]] | ||
[[Category: Xu, J.]] | [[Category: Xu, J.]] | ||
[[Category: bacteriolytic enzyme]] | [[Category: bacteriolytic enzyme]] | ||
[[Category: glycosidase]] | [[Category: glycosidase]] | ||
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[[Category: o-glycosyl]] | [[Category: o-glycosyl]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:33:43 2008'' | ||
Revision as of 20:33, 30 March 2008
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| , resolution 1.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF T4 LYSOZYME MUTANT V149C
OverviewOverview
To investigate the structural and thermodynamic basis of the binding of solvent at internal sites within proteins a number of mutations were constructed in T4 lysozyme. Some of these were designed to introduce new solvent-binding sites. Others were intended to displace solvent from preexisting sites. In one case Val-149 was replaced with alanine, serine, cysteine, threonine, isoleucine, and glycine. Crystallographic analysis shows that, with the exception of isoleucine, each of these substitutions results in the binding of solvent at a polar site that is sterically blocked in the wild-type enzyme. Mutations designed to perturb or displace a solvent molecule present in the native enzyme included the replacement of Thr-152 with alanine, serine, cysteine, valine, and isoleucine. Although the solvent molecule was moved in some cases by up to 1.7 A, in no case was it completely removed from the folded protein. The results suggest that hydrogen bonds from the protein to bound solvent are energy neutral. The binding of solvent to internal sites within proteins also appears to be energy neutral except insofar as the bound solvent may prevent a loss of energy due to potential hydrogen bonding groups that would otherwise be unsatisfied. The introduction of a solvent-binding site appears to require not only a cavity to accommodate the water molecule but also the presence of polar groups to help satisfy its hydrogen-bonding potential. It may be easier to design a site to accommodate two or more water molecules rather than one as the solvent molecules can then hydrogen-bond to each other. For similar reasons it is often difficult to design a point mutation that will displace a single solvent molecule from the core of a protein.
About this StructureAbout this Structure
1G07 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
ReferenceReference
Structural and thermodynamic analysis of the binding of solvent at internal sites in T4 lysozyme., Xu J, Baase WA, Quillin ML, Baldwin EP, Matthews BW, Protein Sci. 2001 May;10(5):1067-78. PMID:11316887
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