1k5j

The Crystal Structure of Nucleoplasmin-CoreThe Crystal Structure of Nucleoplasmin-Core

Structural highlights 1k5j is a 5 chain structure with sequence from African clawed frog. The January 2007 RCSB PDB Molecule of the Month feature on Importins by David S. Goodsell is 10.2210/rcsb_pdb/mom_2007_1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: Nucleoplasmin (African clawed frog) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Function [NUPL_XENLA] Core histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Nucleoplasmin is an acidic protein which is able to assemble nucleosomes by binding histones and transferring them to DNA. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The efficient assembly of histone complexes and nucleosomes requires the participation of molecular chaperones. Currently, there is a paucity of data on their mechanism of action. We now present the structure of an N-terminal domain of nucleoplasmin (Np-core) at 2.3 A resolution. The Np-core monomer is an eight-stranded beta barrel that fits snugly within a stable pentamer. In the crystal, two pentamers associate to form a decamer. We show that both Np and Np-core are competent to assemble large complexes that contain the four core histones. Further experiments and modeling suggest that these complexes each contain five histone octamers which dock to a central Np decamer. This work has important ramifications for models of histone storage, sperm chromatin decondensation, and nucleosome assembly. The crystal structure of nucleoplasmin-core: implications for histone binding and nucleosome assembly.,Dutta S, Akey IV, Dingwall C, Hartman KL, Laue T, Nolte RT, Head JF, Akey CW Mol Cell. 2001 Oct;8(4):841-53. PMID:11684019 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Nucleoplasmin

spinqualitylabelsall models 1k5j, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image