1esm: Difference between revisions
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|PDB= 1esm |SIZE=350|CAPTION= <scene name='initialview01'>1esm</scene>, resolution 2.5Å | |PDB= 1esm |SIZE=350|CAPTION= <scene name='initialview01'>1esm</scene>, resolution 2.5Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=COA:COENZYME A'>COA</scene> | |LIGAND= <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Pantothenate_kinase Pantothenate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.33 2.7.1.33] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantothenate_kinase Pantothenate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.33 2.7.1.33] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1esn|1ESN]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1esm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esm OCA], [http://www.ebi.ac.uk/pdbsum/1esm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1esm RCSB]</span> | |||
}} | }} | ||
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[[Category: Rock, C O.]] | [[Category: Rock, C O.]] | ||
[[Category: Yun, M.]] | [[Category: Yun, M.]] | ||
[[Category: protein-inhibitor complex]] | [[Category: protein-inhibitor complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:08:50 2008'' | ||
Revision as of 20:08, 30 March 2008
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| , resolution 2.5Å | |||||||
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| Ligands: | , | ||||||
| Activity: | Pantothenate kinase, with EC number 2.7.1.33 | ||||||
| Related: | 1ESN
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURAL BASIS FOR THE FEEDBACK REGULATION OF ESCHERICHIA COLI PANTOTHENATE KINASE BY COENZYME A
OverviewOverview
Pantothenate kinase (PanK) is a key regulatory enzyme in the coenzyme A (CoA) biosynthetic pathway and catalyzes the phosphorylation of pantothenic acid to form phosphopantothenate. CoA is a feedback inhibitor of PanK activity by competitive binding to the ATP site. The structures of the Escherichia coli enzyme, in complex with a nonhydrolyzable analogue of ATP, 5'-adenylimido-diphosphate (AMPPNP), or with CoA, were determined at 2.6 and 2.5 A, respectively. Both structures show that two dimers occupy an asymmetric unit; each subunit has a alpha/beta mononucleotide-binding fold with an extensive antiparallel coiled coil formed by two long helices along the dimerization interface. The two ligands, AMPPNP and CoA, associate with PanK in very different ways, but their phosphate binding sites overlap, explaining the kinetic competition between CoA and ATP. Residues Asp(127), His(177), and Arg(243) are proposed to be involved in catalysis, based on modeling of the pentacoordinate transition state. The more potent inhibition by CoA, compared with the CoA thioesters, is explained by a tight interaction of the CoA thiol group with the side chains of aromatic residues, which is predicted to discriminate against the CoA thioesters. The PanK structure provides the framework for a more detailed understanding of the mechanism of catalysis and feedback regulation of PanK.
About this StructureAbout this Structure
1ESM is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for the feedback regulation of Escherichia coli pantothenate kinase by coenzyme A., Yun M, Park CG, Kim JY, Rock CO, Jackowski S, Park HW, J Biol Chem. 2000 Sep 8;275(36):28093-9. PMID:10862768
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