1en7: Difference between revisions
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|PDB= 1en7 |SIZE=350|CAPTION= <scene name='initialview01'>1en7</scene>, resolution 2.4Å | |PDB= 1en7 |SIZE=350|CAPTION= <scene name='initialview01'>1en7</scene>, resolution 2.4Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] </span> | ||
|GENE= GP49 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | |GENE= GP49 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1en7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1en7 OCA], [http://www.ebi.ac.uk/pdbsum/1en7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1en7 RCSB]</span> | |||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
1EN7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | 1EN7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EN7 OCA]. | ||
==Reference== | ==Reference== | ||
X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture., Raaijmakers H, Vix O, Toro I, Golz S, Kemper B, Suck D, EMBO J. 1999 Mar 15;18(6):1447-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10075917 10075917] | X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture., Raaijmakers H, Vix O, Toro I, Golz S, Kemper B, Suck D, EMBO J. 1999 Mar 15;18(6):1447-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10075917 10075917] | ||
[[Category: Crossover junction endodeoxyribonuclease]] | [[Category: Crossover junction endodeoxyribonuclease]] | ||
[[Category: Enterobacteria phage t4]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Raaijmakers, H.]] | [[Category: Raaijmakers, H.]] | ||
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[[Category: Toro, I.]] | [[Category: Toro, I.]] | ||
[[Category: Vix, O.]] | [[Category: Vix, O.]] | ||
[[Category: dnase]] | [[Category: dnase]] | ||
[[Category: endonuclease]] | [[Category: endonuclease]] | ||
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[[Category: resolvase]] | [[Category: resolvase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:05:56 2008'' | ||
Revision as of 20:05, 30 March 2008
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| , resolution 2.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | GP49 (Enterobacteria phage T4) | ||||||
| Activity: | Crossover junction endodeoxyribonuclease, with EC number 3.1.22.4 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ENDONUCLEASE VII (ENDOVII) FROM PHAGE T4
OverviewOverview
Phage T4 endonuclease VII (Endo VII), the first enzyme shown to resolve Holliday junctions, recognizes a broad spectrum of DNA substrates ranging from branched DNAs to single base mismatches. We have determined the crystal structures of the Ca2+-bound wild-type and the inactive N62D mutant enzymes at 2.4 and 2.1 A, respectively. The Endo VII monomers form an elongated, highly intertwined molecular dimer exhibiting extreme domain swapping. The major dimerization elements are two pairs of antiparallel helices forming a novel 'four-helix cross' motif. The unique monomer fold, almost completely lacking beta-sheet structure and containing a zinc ion tetrahedrally coordinated to four cysteines, does not resemble any of the known junction-resolving enzymes, including the Escherichia coli RuvC and lambda integrase-type recombinases. The S-shaped dimer has two 'binding bays' separated by approximately 25 A which are lined by positively charged residues and contain near their base residues known to be essential for activity. These include Asp40 and Asn62, which function as ligands for the bound calcium ions. A pronounced bipolar charge distribution suggests that branched DNA substrates bind to the positively charged face with the scissile phosphates located near the divalent cations. A model for the complex with a four-way DNA junction is presented.
About this StructureAbout this Structure
1EN7 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
ReferenceReference
X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture., Raaijmakers H, Vix O, Toro I, Golz S, Kemper B, Suck D, EMBO J. 1999 Mar 15;18(6):1447-58. PMID:10075917
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