1eaq: Difference between revisions
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|PDB= 1eaq |SIZE=350|CAPTION= <scene name='initialview01'>1eaq</scene>, resolution 1.25Å | |PDB= 1eaq |SIZE=350|CAPTION= <scene name='initialview01'>1eaq</scene>, resolution 1.25Å | ||
|SITE= <scene name='pdbsite=CL1:Chloride+Binding+Site+For+Chain+B'>CL1</scene> | |SITE= <scene name='pdbsite=CL1:Chloride+Binding+Site+For+Chain+B'>CL1</scene> | ||
|LIGAND= <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eaq OCA], [http://www.ebi.ac.uk/pdbsum/1eaq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1eaq RCSB]</span> | |||
}} | }} | ||
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[[Category: Sauer, U H.]] | [[Category: Sauer, U H.]] | ||
[[Category: Wolf-Watz, M.]] | [[Category: Wolf-Watz, M.]] | ||
[[Category: acute myeloid leukemia]] | [[Category: acute myeloid leukemia]] | ||
[[Category: aml]] | [[Category: aml]] | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:58:51 2008'' | ||
Revision as of 19:58, 30 March 2008
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| , resolution 1.25Å | |||||||
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| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE RUNX1 RUNT DOMAIN AT 1.25A RESOLUTION: A STRUCTURAL SWITCH AND SPECIFICALLY BOUND CHLORIDE IONS MODULATE DNA BINDING
OverviewOverview
The evolutionarily conserved Runt homology domain is characteristic of the RUNX family of heterodimeric eukaryotic transcription factors, including RUNX1, RUNX2 and RUNX3. The genes for RUNX1, also termed acute myeloid leukemia protein 1, AML1, and its dimerization partner core-binding factor beta, CBFbeta, are essential for hematopoietic development and are together the most common targets for gene rearrangements in acute human leukemias. Here, we describe the crystal structure of the uncomplexed RUNX1 Runt domain at 1.25A resolution and compare its conformation to previously published structures in complex with DNA, CBFbeta or both. We find that complex formation induces significant structural rearrangements in this immunoglobulin (Ig)-like DNA-binding domain. Most pronounced is the movement of loop L11, which changes from a closed conformation in the free Runt structure to an open conformation in the CBFbeta-bound and DNA-bound forms. This transition, which we refer to as the S-switch, and accompanying structural movements that affect other parts of the Runt domain are crucial for sustained DNA binding. The closed to open transition can be induced by CBFbeta alone; suggesting that one role of CBFbeta is to trigger the S-switch and to stabilize the Runt domain in a conformation enhanced for DNA binding.A feature of the Runt domain hitherto unobserved in any Ig-like DNA-binding domain is the presence of two specifically bound chloride ions. One chloride ion is coordinated by amino acid residues that make direct DNA contact. In a series of electrophoretic mobility-shift analyses, we demonstrate a chloride ion concentration-dependent stimulation of the DNA-binding activity of Runt in the physiological range. A comparable DNA-binding stimulation was observed for negatively charged amino acid residues. This suggests a regulatory mechanism of RUNX proteins through acidic amino acid residues provided by activation domains during cooperative interaction with other transcription factors.
About this StructureAbout this Structure
1EAQ is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
The RUNX1 Runt domain at 1.25A resolution: a structural switch and specifically bound chloride ions modulate DNA binding., Backstrom S, Wolf-Watz M, Grundstrom C, Hard T, Grundstrom T, Sauer UH, J Mol Biol. 2002 Sep 13;322(2):259-72. PMID:12217689
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