2cii: Difference between revisions
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==Overview== | ==Overview== | ||
In the absence of bound peptide ligands, major histocompatibility complex, (MHC) class I molecules are unstable. In an attempt to determine the, minimum requirement for peptide-dependent MHC class I stabilization, we, have used short synthetic peptides derived from the Sendai virus, nucleoprotein epitope (residues 324-332, 1FAPGNYPAL9) to promote its, folding in vitro of H-2D(b). We found that H-2D(b) can be stabilized by, the pentapeptide 5NYPAL9, which is equivalent to the C-terminal portion of, the optimal nonapeptide and includes both the P5 and P9 anchor residues., We have crystallized the complex of the H-2D(b) molecule with the pentamer, and determined the structure to show how a quasi-stable MHC class I, molecule can be formed by occupancy of a single binding pocket in the, . | In the absence of bound peptide ligands, major histocompatibility complex, (MHC) class I molecules are unstable. In an attempt to determine the, minimum requirement for peptide-dependent MHC class I stabilization, we, have used short synthetic peptides derived from the Sendai virus, nucleoprotein epitope (residues 324-332, 1FAPGNYPAL9) to promote its, folding in vitro of H-2D(b). We found that H-2D(b) can be stabilized by, the pentapeptide 5NYPAL9, which is equivalent to the C-terminal portion of, the optimal nonapeptide and includes both the P5 and P9 anchor residues., We have crystallized the complex of the H-2D(b) molecule with the pentamer, and determined the structure to show how a quasi-stable MHC class I, molecule can be formed by occupancy of a single binding pocket in the, peptide-binding groove. | ||
==About this Structure== | ==About this Structure== | ||
2CII is a | 2CII is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CII OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:17:05 2007'' | ||
Revision as of 16:11, 5 November 2007
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THE CRYSTAL STRUCTURE OF H-2DB COMPLEXED WITH A PARTIAL PEPTIDE EPITOPE SUGGESTS AN MHC CLASS I ASSEMBLY-INTERMEDIATE
OverviewOverview
In the absence of bound peptide ligands, major histocompatibility complex, (MHC) class I molecules are unstable. In an attempt to determine the, minimum requirement for peptide-dependent MHC class I stabilization, we, have used short synthetic peptides derived from the Sendai virus, nucleoprotein epitope (residues 324-332, 1FAPGNYPAL9) to promote its, folding in vitro of H-2D(b). We found that H-2D(b) can be stabilized by, the pentapeptide 5NYPAL9, which is equivalent to the C-terminal portion of, the optimal nonapeptide and includes both the P5 and P9 anchor residues., We have crystallized the complex of the H-2D(b) molecule with the pentamer, and determined the structure to show how a quasi-stable MHC class I, molecule can be formed by occupancy of a single binding pocket in the, peptide-binding groove.
About this StructureAbout this Structure
2CII is a Protein complex structure of sequences from Homo sapiens and Mus musculus with GOL as ligand. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of H-2D(b) complexed with a partial peptide epitope suggests a major histocompatibility complex class I assembly intermediate., Glithero A, Tormo J, Doering K, Kojima M, Jones EY, Elliott T, J Biol Chem. 2006 May 5;281(18):12699-704. Epub 2006 Feb 14. PMID:16478731
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