2aff

The solution structure of the Ki67FHA/hNIFK(226-269)3P complexThe solution structure of the Ki67FHA/hNIFK(226-269)3P complex

Structural highlights 2aff is a 2 chain structure with sequence from Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: , Related: 1r21 Gene: MKI67 (HUMAN), MKI67IP (HUMAN) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Warning: this is a large structure, and loading might take a long time or not happen at all. Function [KI67_HUMAN] Thought to be required for maintaining cell proliferation. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The forkhead-associated (FHA) domain of human Ki67 interacts with the human nucleolar protein hNIFK, recognizing a 44-residue fragment, hNIFK226-269, phosphorylated at Thr234. Here we show that high-affinity binding requires sequential phosphorylation by two kinases, CDK1 and GSK3, yielding pThr238, pThr234 and pSer230. We have determined the solution structure of Ki67FHA in complex with the triply phosphorylated peptide hNIFK226-269(3P), revealing not only local recognition of pThr234 but also the extension of the beta-sheet of the FHA domain by the addition of a beta-strand of hNIFK. The structure of an FHA domain in complex with a biologically relevant binding partner provides insights into ligand specificity and potentially links the cancer marker protein Ki67 to a signaling pathway associated with cell fate specification. Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67.,Byeon IJ, Li H, Song H, Gronenborn AM, Tsai MD Nat Struct Mol Biol. 2005 Nov;12(11):987-93. PMID:16244663 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 2aff, 100 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image