1d3c: Difference between revisions
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|PDB= 1d3c |SIZE=350|CAPTION= <scene name='initialview01'>1d3c</scene>, resolution 1.78Å | |PDB= 1d3c |SIZE=350|CAPTION= <scene name='initialview01'>1d3c</scene>, resolution 1.78Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1cdg|1CDG]], [[1cxi|1CXI]], [[1cxf|1CXF]], [[1cxk|1CXK]], [[1cxl|1CXL]], [[2dij|2DIJ]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d3c OCA], [http://www.ebi.ac.uk/pdbsum/1d3c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d3c RCSB]</span> | |||
}} | }} | ||
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[[Category: Uitdehaag, J C.M.]] | [[Category: Uitdehaag, J C.M.]] | ||
[[Category: Veen, B A.van der.]] | [[Category: Veen, B A.van der.]] | ||
[[Category: alpha-amylase]] | [[Category: alpha-amylase]] | ||
[[Category: catalysis]] | [[Category: catalysis]] | ||
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[[Category: transglycosylation]] | [[Category: transglycosylation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:33:48 2008'' | ||
Revision as of 19:33, 30 March 2008
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| , resolution 1.78Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Activity: | Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 | ||||||
| Related: | 1CDG, 1CXI, 1CXF, 1CXK, 1CXL, 2DIJ
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
MICHAELIS COMPLEX OF BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE WITH GAMMA-CYCLODEXTRIN
OverviewOverview
The enzyme cyclodextrin glycosyltransferase is closely related to alpha-amylases but has the unique ability to produce cyclodextrins (circular alpha(1-->4)-linked glucoses) from starch. To characterize this specificity we determined a 1.8-A structure of an E257Q/D229N mutant cyclodextrin glycosyltransferase in complex with its product gamma-cyclodextrin, which reveals for the first time how cyclodextrin is competently bound. Across subsites -2, -1, and +1, the cyclodextrin ring binds in a twisted mode similar to linear sugars, giving rise to deformation of its circular symmetry. At subsites -3 and +2, the cyclodextrin binds in a manner different from linear sugars. Sequence comparisons and site-directed mutagenesis experiments support the conclusion that subsites -3 and +2 confer the cyclization activity in addition to subsite -6 and Tyr-195. On this basis, a role of the individual residues during the cyclization reaction cycle is proposed.
About this StructureAbout this Structure
1D3C is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.
ReferenceReference
The cyclization mechanism of cyclodextrin glycosyltransferase (CGTase) as revealed by a gamma-cyclodextrin-CGTase complex at 1.8-A resolution., Uitdehaag JC, Kalk KH, van Der Veen BA, Dijkhuizen L, Dijkstra BW, J Biol Chem. 1999 Dec 3;274(49):34868-76. PMID:10574960
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