1crx: Difference between revisions
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|PDB= 1crx |SIZE=350|CAPTION= <scene name='initialview01'>1crx</scene>, resolution 2.4Å | |PDB= 1crx |SIZE=350|CAPTION= <scene name='initialview01'>1crx</scene>, resolution 2.4Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= CRE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | |GENE= CRE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10678 Enterobacteria phage P1]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1crx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crx OCA], [http://www.ebi.ac.uk/pdbsum/1crx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1crx RCSB]</span> | |||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
1CRX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | 1CRX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p1 Enterobacteria phage p1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRX OCA]. | ||
==Reference== | ==Reference== | ||
Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse., Guo F, Gopaul DN, van Duyne GD, Nature. 1997 Sep 4;389(6646):40-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9288963 9288963] | Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse., Guo F, Gopaul DN, van Duyne GD, Nature. 1997 Sep 4;389(6646):40-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9288963 9288963] | ||
[[Category: Enterobacteria phage | [[Category: Enterobacteria phage p1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Duyne, G D.Van.]] | [[Category: Duyne, G D.Van.]] | ||
[[Category: Gopaul, D N.]] | [[Category: Gopaul, D N.]] | ||
[[Category: Guo, F.]] | [[Category: Guo, F.]] | ||
[[Category: complex (recombinase/dna)]] | [[Category: complex (recombinase/dna)]] | ||
[[Category: protein:dna complex]] | [[Category: protein:dna complex]] | ||
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[[Category: site-specific recombinase]] | [[Category: site-specific recombinase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:27:27 2008'' | ||
Revision as of 19:27, 30 March 2008
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| , resolution 2.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Gene: | CRE (Enterobacteria phage P1) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I
OverviewOverview
During site-specific DNA recombination, which brings about genetic rearrangement in processes such as viral integration and excision and chromosomal segregation, recombinase enzymes recognize specific DNA sequences and catalyse the reciprocal exchange of DNA strands between these sites. The bacteriophage recombinase Cre catalyses site-specific recombination between two 34-base-pair loxP sites. The crystal structure at 2.4 A resolution of Cre bound to a loxP substrate reveals an intermediate in the recombination reaction, in which a Cre molecule has cleaved the substrate to form a covalent 3'-phosphotyrosine linkage with the DNA. Four recombinases and two loxP sites form a synapsed structure in which the DNA resembles models of four-way Holliday-Junction intermediates. The Cre-loxP complex challenges models of site-specific recombination that require large changes in quaternary structure. Subtle allosteric changes at the carboxy termini of the Cre subunits may instead coordinate the cleavage and strand-exchange reactions.
About this StructureAbout this Structure
1CRX is a Single protein structure of sequence from Enterobacteria phage p1. Full crystallographic information is available from OCA.
ReferenceReference
Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse., Guo F, Gopaul DN, van Duyne GD, Nature. 1997 Sep 4;389(6646):40-6. PMID:9288963
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