ConSurf Quick Analysis Procedure: Difference between revisions
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# Enter your [[PDB code]] and click the ''Next'' button. | # Enter your [[PDB code]] and click the ''Next'' button. | ||
# Choose one chain from the pull-down ''Chain Identifier'' menu. (ConSurf can only analyze [[Conservation%2C_Evolutionary#Use_Caution_When_Comparing_Conservation_of_Sequence-Different_Chains|one chain at a time]].) | # Choose one chain from the pull-down ''Chain Identifier'' menu. (ConSurf can only analyze [[Conservation%2C_Evolutionary#Use_Caution_When_Comparing_Conservation_of_Sequence-Different_Chains|one chain at a time]].) | ||
# | # Check '''No''' you do not have a Multiple Sequence Alignment (MSA) to upload. | ||
# Don't worry, you can leave all the settings that appear now at their defaults. | |||
Revision as of 01:28, 7 February 2016
Here is a quick procedure for coloring a protein molecule by evolutionary conservation. This procedure does not necessarily give the highest quality result, but will give you a preliminary result that is often sufficient. If you wish to take it further, see Limiting ConSurf Analysis to Proteins of a Single Function.
- Go to the ConSurf Server.
- Check Amino Acids.
- Check Yes there is a known protein structure.
- Enter your PDB code and click the Next button.
- Choose one chain from the pull-down Chain Identifier menu. (ConSurf can only analyze one chain at a time.)
- Check No you do not have a Multiple Sequence Alignment (MSA) to upload.
- Don't worry, you can leave all the settings that appear now at their defaults.