Dronpa: Difference between revisions
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<StructureSection load='2iov' size=' | <StructureSection load='2iov' size='350' side='right' scene='33/330991/Cv/1' caption='Fluorescent protein Dronpa complex with CYG chromophore [[2iov]]'> | ||
=== Function=== | === Function=== | ||
'''Dronpa''' is a [[:Category:Gfp-like protein|GFP]]-like photoswitchable protein. It was first designed by Ryoko Ando, Hideaki Mizuno, and Atsushi Miyawaki in 2004. The protein was named after the “Japanese term Dron, a ninja term referring to instant disappearance of the body, and pa, short for photo activation.”<ref name="webcite"> [http://www.brain.riken.jp/bsi-news/bsinews29/no29/research3e.html] (retrieved on November 30, 2008), </ref> Using a laser diode at 405nm the protein can be activated into its “bright” state, and using an argon laser at 480nm, the protein can be deactivated and turned into its “dark” state<ref name="webcite" />. Dronpa can be turned back and forth as often as necessary just by switching the wavelength of light that is focused onto it. Dronpa works by switching between the cis (bright state) to trans (dark state) position of a few amino acids in the chromophore center of the <scene name='33/330991/Cv/2'>Beta-barrel</scene> structure. This reversible ability to be able to switch back and forth between “dark” and “light” is what makes Dronpa unique. | '''Dronpa''' is a [[:Category:Gfp-like protein|GFP]]-like photoswitchable protein. It was first designed by Ryoko Ando, Hideaki Mizuno, and Atsushi Miyawaki in 2004. The protein was named after the “Japanese term Dron, a ninja term referring to instant disappearance of the body, and pa, short for photo activation.”<ref name="webcite"> [http://www.brain.riken.jp/bsi-news/bsinews29/no29/research3e.html] (retrieved on November 30, 2008), </ref> Using a laser diode at 405nm the protein can be activated into its “bright” state, and using an argon laser at 480nm, the protein can be deactivated and turned into its “dark” state<ref name="webcite" />. Dronpa can be turned back and forth as often as necessary just by switching the wavelength of light that is focused onto it. Dronpa works by switching between the cis (bright state) to trans (dark state) position of a few amino acids in the chromophore center of the <scene name='33/330991/Cv/2'>Beta-barrel</scene> structure. This reversible ability to be able to switch back and forth between “dark” and “light” is what makes Dronpa unique. See [[Colored & Bioluminescent Protein]]. | ||
=== Structural highlights=== | === Structural highlights=== | ||