1bzx: Difference between revisions
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|PDB= 1bzx |SIZE=350|CAPTION= <scene name='initialview01'>1bzx</scene>, resolution 2.1Å | |PDB= 1bzx |SIZE=350|CAPTION= <scene name='initialview01'>1bzx</scene>, resolution 2.1Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bzx OCA], [http://www.ebi.ac.uk/pdbsum/1bzx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bzx RCSB]</span> | |||
}} | }} | ||
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[[Category: Smalas, A O.]] | [[Category: Smalas, A O.]] | ||
[[Category: Willassen, N P.]] | [[Category: Willassen, N P.]] | ||
[[Category: cold adaptation]] | [[Category: cold adaptation]] | ||
[[Category: inhibitor]] | [[Category: inhibitor]] | ||
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[[Category: trypsin]] | [[Category: trypsin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:11:45 2008'' | ||
Revision as of 19:11, 30 March 2008
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| , resolution 2.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Trypsin, with EC number 3.4.21.4 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE CRYSTAL STRUCTURE OF ANIONIC SALMON TRYPSIN IN COMPLEX WITH BOVINE PANCREATIC TRYPSIN INHIBITOR
OverviewOverview
The complex formed between anionic salmon trypsin (ST) and bovine pancreatic trypsin inhibitor (BPTI) has been crystallised, and the X-ray structure has been solved using the molecular replacement method. The crystals are hexagonal and belong to space group P6(1)22 with lattice parameters of a = b = 83.12 A and c = 222.15 A. Data have been collected to 2.1 A and the structure has been refined to a crystallographic R-factor of 20.6%. Catalysis by salmon trypsin is distinguished by a Km value 20-fold lower than that for mammalian trypsins, and a k(cat) twice as high. The present ST-BPTI complex serves as a model for the Michaelis-Menten complex, and has been compared with corresponding bovine and rat trypsin (RT) complexes. The binding of BPTI to salmon trypsin is characterised by stronger primary interactions in the active site, and a somewhat looser secondary binding.
About this StructureAbout this Structure
1BZX is a Protein complex structure of sequences from Bos taurus and Salmo salar. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of anionic salmon trypsin in complex with bovine pancreatic trypsin inhibitor., Helland R, Leiros I, Berglund GI, Willassen NP, Smalas AO, Eur J Biochem. 1998 Sep 1;256(2):317-24. PMID:9760170
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