The Bacterial Flagellar Hook: Difference between revisions
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===FlgE31 2004=== | ===FlgE31 2004=== | ||
<applet load='1wlg' size=' | <applet load='1wlg' size='350' frame='true' align='right' scene='The_Bacterial_Flagellar_Hook/1wlg_1_mmol/3' caption='Salmonella typhimurium flagellar hook protein FLGE [[1wlg]]'/> | ||
In 2004, Samatey ''et al.'' solved the structure of the mid-portion of the wild-type FlgE of ''Salmonella typhimurium'' by [[X-ray crystallography]] at a [[Resolution|resolution]] of 1.8 Å ([[1wlg]])<ref name="hook1">PMID: 15510139</ref>. The fragment successfully crystallized, designated FlgE31, consisted of amino acids 71-369, of which 71-363 were resolved (72% of the full-length 402-residue protein). Removal of the ends of the full-length chain (domain D0, see below) was required in order to coax the protein to crystallize, instead of forming filaments. | In 2004, Samatey ''et al.'' solved the structure of the mid-portion of the wild-type FlgE of ''Salmonella typhimurium'' by [[X-ray crystallography]] at a [[Resolution|resolution]] of 1.8 Å ([[1wlg]])<ref name="hook1">PMID: 15510139</ref>. The fragment successfully crystallized, designated FlgE31, consisted of amino acids 71-369, of which 71-363 were resolved (72% of the full-length 402-residue protein). Removal of the ends of the full-length chain (domain D0, see below) was required in order to coax the protein to crystallize, instead of forming filaments. | ||
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==Monomer Within the Hook== | ==Monomer Within the Hook== | ||
<applet size=' | <applet size='350' frame='true' align='right' scene='The_Bacterial_Flagellar_Hook/Flagellar_hook_3rings_pdb_gz/3'/> | ||
From the curved model of the hook, here is a short segment just three Flg31 monomers long (<scene name='The_Bacterial_Flagellar_Hook/Flagellar_hook_3rings_pdb_gz/3'>restore initial scene</scene>). Each color represents one of the 11 protofilaments. Since each protofilament is 3 monomers in length, there are 33 monomers in this model. You can see how the Flg31 monomers fit into the hook when <scene name='The_Bacterial_Flagellar_Hook/Flagellar_hook_3rings_pdb_gz/2'>two monomers are highlighted</scene>. | From the curved model of the hook, here is a short segment just three Flg31 monomers long (<scene name='The_Bacterial_Flagellar_Hook/Flagellar_hook_3rings_pdb_gz/3'>restore initial scene</scene>). Each color represents one of the 11 protofilaments. Since each protofilament is 3 monomers in length, there are 33 monomers in this model. You can see how the Flg31 monomers fit into the hook when <scene name='The_Bacterial_Flagellar_Hook/Flagellar_hook_3rings_pdb_gz/2'>two monomers are highlighted</scene>. | ||