1bs1: Difference between revisions
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|PDB= 1bs1 |SIZE=350|CAPTION= <scene name='initialview01'>1bs1</scene>, resolution 1.8Å | |PDB= 1bs1 |SIZE=350|CAPTION= <scene name='initialview01'>1bs1</scene>, resolution 1.8Å | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=DAA:8-AMINO-7-CARBOXYAMINO-NONANOIC+ACID+WITH+ALUMINUM+FLUORIDE'>DAA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Dethiobiotin_synthase Dethiobiotin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.3.3 6.3.3.3] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Dethiobiotin_synthase Dethiobiotin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.3.3 6.3.3.3] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bs1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bs1 OCA], [http://www.ebi.ac.uk/pdbsum/1bs1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bs1 RCSB]</span> | |||
}} | }} | ||
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[[Category: Sandmark, J.]] | [[Category: Sandmark, J.]] | ||
[[Category: Schneider, G.]] | [[Category: Schneider, G.]] | ||
[[Category: aluminum flouride]] | [[Category: aluminum flouride]] | ||
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
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[[Category: phosphoryl transfer]] | [[Category: phosphoryl transfer]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:07:12 2008'' | ||
Revision as of 19:07, 30 March 2008
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| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Activity: | Dethiobiotin synthase, with EC number 6.3.3.3 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DETHIOBIOTIN SYNTHETASE COMPLEXED WITH DETHIOBIOTIN, ADP , INORGANIC PHOSPHATE AND MAGNESIUM
OverviewOverview
The crystal structures of two complexes of dethiobiotin synthetase, enzyme-diaminopelargonic acid-MgADP-AlF3 and enzyme-dethiobiotin-MgADP-Pi, respectively, have been determined to 1.8 A resolution. In dethiobiotin synthetase, AlF3 together with carbamylated diaminopelargonic acid mimics the phosphorylated reaction intermediate rather than the transition state complex for phosphoryl transfer. Observed differences in the binding of substrate, diaminopelargonic acid, and the product, dethiobiotin, suggest considerable displacements of substrate atoms during the ring closure step of the catalytic reaction. In both complexes, two metal ions are observed at the active site, providing evidence for a two-metal mechanism for this enzyme.
About this StructureAbout this Structure
1BS1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of two quaternary complexes of dethiobiotin synthetase, enzyme-MgADP-AlF3-diaminopelargonic acid and enzyme-MgADP-dethiobiotin-phosphate; implications for catalysis., Kack H, Sandmark J, Gibson KJ, Schneider G, Lindqvist Y, Protein Sci. 1998 Dec;7(12):2560-6. PMID:9865950
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