4uxf: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
No edit summary
No edit summary
Line 1: Line 1:
'''Unreleased structure'''
==Crystal structure of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34, P21 native crystal==
<StructureSection load='4uxf' size='340' side='right' caption='[[4uxf]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4uxf]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UXF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UXF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uxe|4uxe]], [[4uxg|4uxg]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uxf OCA], [http://pdbe.org/4uxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uxf RCSB], [http://www.ebi.ac.uk/pdbsum/4uxf PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/FIBP_BPT4 FIBP_BPT4]] Structural component of the proximal-half of the long-tail fiber. The long-tail fibers of T4 are about 1600 Angstroms long with a kink in the middle that divides the fiber into proximal and distal halves.<ref>PMID:8709154</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The phage-proximal part of the long tail fibres of bacteriophage T4 consists of a trimer of the 1289 amino-acid gene product 34 (gp34). Different carboxy-terminal parts of gp34 have been produced and crystallized. Crystals of gp34(726-1289) diffracting X-rays to 2.9 A resolution, crystals of gp34(781-1289) diffracting to 1.9 A resolution and crystals of gp34(894-1289) diffracting to 3.0 and 2.0 A resolution and belonging to different crystal forms were obtained. Native data were collected for gp34(726-1289) and gp34(894-1289), while single-wavelength anomalous diffraction data were collected for selenomethionine-containing gp34(781-1289) and gp34(894-1289). For the latter, high-quality anomalous signal was obtained.


The entry 4uxf is ON HOLD  until Paper Publication
Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34.,Granell M, Namura M, Alvira S, Garcia-Doval C, Singh AK, Gutsche I, van Raaij MJ, Kanamaru S Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):970-5. doi:, 10.1107/S2053230X14010449. Epub 2014 Jun 19. PMID:25005101<ref>PMID:25005101</ref>


Authors: Granell, M., Alvira, S., Garcia-Doval, C., Singh, A.K., van Raaij, M.J.
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
Description: Crystal structure of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34, P21 native crystal
<div class="pdbe-citations 4uxf" style="background-color:#fffaf0;"></div>
[[Category: Unreleased Structures]]
== References ==
[[Category: Granell, M]]
<references/>
[[Category: Van Raaij, M.J]]
__TOC__
</StructureSection>
[[Category: Alvira, S]]
[[Category: Alvira, S]]
[[Category: Singh, A.K]]
[[Category: Garcia-Doval, C]]
[[Category: Garcia-Doval, C]]
[[Category: Granell, M]]
[[Category: Raaij, M J.van]]
[[Category: Singh, A K]]
[[Category: Caudovirale]]
[[Category: Myoviridae]]
[[Category: Triple beta-helix]]
[[Category: Viral protein]]

Revision as of 16:47, 16 December 2015

Crystal structure of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34, P21 native crystalCrystal structure of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34, P21 native crystal

Structural highlights

4uxf is a 3 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[FIBP_BPT4] Structural component of the proximal-half of the long-tail fiber. The long-tail fibers of T4 are about 1600 Angstroms long with a kink in the middle that divides the fiber into proximal and distal halves.[1]

Publication Abstract from PubMed

The phage-proximal part of the long tail fibres of bacteriophage T4 consists of a trimer of the 1289 amino-acid gene product 34 (gp34). Different carboxy-terminal parts of gp34 have been produced and crystallized. Crystals of gp34(726-1289) diffracting X-rays to 2.9 A resolution, crystals of gp34(781-1289) diffracting to 1.9 A resolution and crystals of gp34(894-1289) diffracting to 3.0 and 2.0 A resolution and belonging to different crystal forms were obtained. Native data were collected for gp34(726-1289) and gp34(894-1289), while single-wavelength anomalous diffraction data were collected for selenomethionine-containing gp34(781-1289) and gp34(894-1289). For the latter, high-quality anomalous signal was obtained.

Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34.,Granell M, Namura M, Alvira S, Garcia-Doval C, Singh AK, Gutsche I, van Raaij MJ, Kanamaru S Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):970-5. doi:, 10.1107/S2053230X14010449. Epub 2014 Jun 19. PMID:25005101[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Cerritelli ME, Wall JS, Simon MN, Conway JF, Steven AC. Stoichiometry and domainal organization of the long tail-fiber of bacteriophage T4: a hinged viral adhesin. J Mol Biol. 1996 Aug 2;260(5):767-80. PMID:8709154 doi:http://dx.doi.org/10.1006/jmbi.1996.0436
  2. Granell M, Namura M, Alvira S, Garcia-Doval C, Singh AK, Gutsche I, van Raaij MJ, Kanamaru S. Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34. Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):970-5. doi:, 10.1107/S2053230X14010449. Epub 2014 Jun 19. PMID:25005101 doi:http://dx.doi.org/10.1107/S2053230X14010449

4uxf, resolution 2.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA