Laccase: Difference between revisions
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<applet load='1W6L' size='400' frame='true' align=|right| CAPTION='CotA laccase complex with glycerol, O2 and Cu+2 (orange), [[1w6l]]' /> | <applet load='1W6L' size='400' frame='true' align=|right| CAPTION='CotA laccase complex with glycerol, O2 and Cu+2 (orange), [[1w6l]]' /> | ||
== Function == | |||
'''CotA laccase''' belong to the multi-copper oxidase family. | '''CotA laccase''' belong to the multi-copper oxidase family. | ||
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EC 1.10.3.2), ascorbate oxidase (L-ascorbate oxygen | EC 1.10.3.2), ascorbate oxidase (L-ascorbate oxygen | ||
oxidoreductase, EC 1.10.3.3) and ceruloplasmin (Fe(II) oxygen | oxidoreductase, EC 1.10.3.3) and ceruloplasmin (Fe(II) oxygen | ||
oxidoreductase, EC 1.16.3.1). Similar to the other laccases the three dimensional | oxidoreductase, EC 1.16.3.1). Similar to the other laccases the three dimensional structure of CotA [[1w6l]] comprises three cupredoxin domains and four copper ions organised in <scene name='CotA_laccase/Copper_centers/5'>Two copper centers</scene>: | ||
a <scene name='CotA_laccase/Mononuclear_t1copper/1'>mononuclear blue type 1 copper center</scene> and <scene name='CotA_laccase/Copper_centers/7'>a trinuclear center</scene> | a <scene name='CotA_laccase/Mononuclear_t1copper/1'>mononuclear blue type 1 copper center</scene> and <scene name='CotA_laccase/Copper_centers/7'>a trinuclear center</scene><ref>PMID:11514528</ref> | ||
== Structural highlights == | |||
The trinuclear center has two type 3 copper ions, that can be anti-ferromagnetically | The trinuclear center has two type 3 copper ions, that can be anti-ferromagnetically | ||
coupled through an hydroxyl moiety in between them, and one | coupled through an hydroxyl moiety in between them, and one | ||
type 2 copper ion.‡ The mononuclear copper is able to accept an | type 2 copper ion.‡ The mononuclear copper is able to accept an | ||
electron | electron from a variety of phenolic substrates and then transmit | ||
it to the trinuclear centre. | it to the trinuclear centre. | ||
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}} | }} | ||
See [[Blue copper oxidase CueO]] | See [[Blue copper oxidase CueO]] | ||
== References == | |||
<references/> | |||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||