DNA Polymerase in Thermococcus gorgonarius: Difference between revisions
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==DNA Polymerase== | ==DNA Polymerase== | ||
<StructureSection load='1TGO' size='340' side='right' caption='DNA Polymerase | <StructureSection load='1TGO' size='340' side='right' caption='DNA Polymerase from ''Thermococcus gorgonarius'' ' scene=''> | ||
== Function == | == Function == | ||
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== Structural Highlights == | == Structural Highlights == | ||
DNA polymerase from Thermococcus gorgonarius (''Tgo'') is composed of 2,319 nucleotides. Tgo a single polypeptide chain made up of 773 amino acids which are folded into five domains. The five domains in Tgo are the N-terminal, 3’ to 5’ exonuclease, palm, fingers, and the thumb. These five domain are arranged to form a ring shaped molecule. The structure of DNA polymerase can be described by using the “right hand model” which has a <scene name='71/713469/Palmthumbfinger/1'>“palm”, “fingers”, and “thumb” regions</scene>. The palm region (blue) catalyzes the phosphoric transfer. The finger region (green) interacts with the newly incoming nucleotides and the template bases that are paired together with the nucleotides. The thumb (red) helps position the DNA and with translocation. <ref>PMCID:PMC22340</ref> | DNA polymerase from ''Thermococcus gorgonarius'' (''Tgo'') is composed of 2,319 nucleotides. Tgo a single polypeptide chain made up of 773 amino acids which are folded into five domains. The five domains in Tgo are the N-terminal, 3’ to 5’ exonuclease, palm, fingers, and the thumb. These five domain are arranged to form a ring shaped molecule. The structure of DNA polymerase can be described by using the “right hand model” which has a <scene name='71/713469/Palmthumbfinger/1'>“palm”, “fingers”, and “thumb” regions</scene>. The palm region (blue) catalyzes the phosphoric transfer. The finger region (green) interacts with the newly incoming nucleotides and the template bases that are paired together with the nucleotides. The thumb (red) helps position the DNA and with translocation. <ref>PMCID:PMC22340</ref> | ||
== Stabilization == | == Stabilization == | ||
''Thermococcus gorgonarius'' (''Tgo'') is a very thermophilic molecule with a growth range between 55-95 degrees Celsius. In order to replicate properly, the DNA polymerase must be stable and able to tolerate binding to the substrate DNA. ''Tgo'' polymerase contains 41% helix, 22% beta-stands, and 19% turns and also has an increase in hydrogen bonding. | ''Thermococcus gorgonarius'' (''Tgo'') is a very thermophilic molecule with a growth range between 55-95 degrees Celsius. In order to replicate properly, the DNA polymerase must be stable and able to tolerate binding to the substrate DNA. ''Tgo'' polymerase contains 41% helix, 22% beta-stands, and 19% turns and also has an increase in hydrogen bonding. | ||