DNA Polymerase in Thermococcus gorgonarius: Difference between revisions
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DNA polymerase from Thermococcus gorgonarius (Tgo) is composed of 2,319 nucleotides. Tgo a single polypeptide chain made up of 773 amino acids which are folded into five domains. The five domains in Tgo are the N-terminal, 3’ to 5’ exonuclease, palm, fingers, and the thumb. These five domain are arranged to form a ring shaped molecule. The structure of DNA polymerase can be described by using the “right hand model” which has a <scene name='71/713469/Palmthumbfinger/1'>“palm”, “fingers”, and “thumb” regions</scene>. The palm region (blue) catalyzes the phosphoric transfer. The finger region (green) interacts with the newly incoming nucleotides and the template bases that are paired together with the nucleotides. The thumb (red) helps position the DNA and with translocation. <ref>PMCID:PMC22340</ref> | DNA polymerase from Thermococcus gorgonarius (Tgo) is composed of 2,319 nucleotides. Tgo a single polypeptide chain made up of 773 amino acids which are folded into five domains. The five domains in Tgo are the N-terminal, 3’ to 5’ exonuclease, palm, fingers, and the thumb. These five domain are arranged to form a ring shaped molecule. The structure of DNA polymerase can be described by using the “right hand model” which has a <scene name='71/713469/Palmthumbfinger/1'>“palm”, “fingers”, and “thumb” regions</scene>. The palm region (blue) catalyzes the phosphoric transfer. The finger region (green) interacts with the newly incoming nucleotides and the template bases that are paired together with the nucleotides. The thumb (red) helps position the DNA and with translocation. <ref>PMCID:PMC22340</ref> | ||
There are two separate <scene name='71/713469/Disulfidebond/2'>disulfide bonds</scene> are formed at two different cysteine pairs in Thermococcus gorgonarius. Each sulfide atom is shown in yellow. Both of the cysteines are located on the palm domain. One disulfide bonds between cysteine (428) and cysteine (442) stabilize the molecule at one of the folds between a helix in the palm domain and a helix located in the finger domain. While the other disulfide bond created by cysteine (506) and cysteine (509) create more stabilization for the helix in the palm domain at a helical turn. With these disulfide bond this molecule is more thermostable. | There are two separate <scene name='71/713469/Disulfidebond/2'>disulfide bonds</scene> are formed at two different cysteine pairs in Thermococcus gorgonarius. Each sulfide atom is shown in yellow. Both of the cysteines are located on the palm domain. One disulfide bonds between cysteine (428) and cysteine (442) stabilize the molecule at one of the folds between a helix in the palm domain and a helix located in the finger domain. While the other disulfide bond created by cysteine (506) and cysteine (509) create more stabilization for the helix in the palm domain at a helical turn. Sulfurs can withstand high temperature because their melting point about 115 degrees Celsius. With these disulfide bond and structural folding this molecule is more thermostable. | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||