1a77: Difference between revisions
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|PDB= 1a77 |SIZE=350|CAPTION= <scene name='initialview01'>1a77</scene>, resolution 2.00Å | |PDB= 1a77 |SIZE=350|CAPTION= <scene name='initialview01'>1a77</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a77 OCA], [http://www.ebi.ac.uk/pdbsum/1a77 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a77 RCSB]</span> | |||
}} | }} | ||
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[[Category: Hwang, K Y.]] | [[Category: Hwang, K Y.]] | ||
[[Category: Kim, H.]] | [[Category: Kim, H.]] | ||
[[Category: 5'-3' exo/endo nuclease]] | [[Category: 5'-3' exo/endo nuclease]] | ||
[[Category: dna repair]] | [[Category: dna repair]] | ||
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[[Category: rth]] | [[Category: rth]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:34:45 2008'' | ||
Revision as of 18:34, 30 March 2008
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII
OverviewOverview
Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 A resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.
About this StructureAbout this Structure
1A77 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of flap endonuclease-1 from Methanococcus jannaschii., Hwang KY, Baek K, Kim HY, Cho Y, Nat Struct Biol. 1998 Aug;5(8):707-13. PMID:9699635
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