1a55: Difference between revisions
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|PDB= 1a55 |SIZE=350|CAPTION= <scene name='initialview01'>1a55</scene>, resolution 2.4Å | |PDB= 1a55 |SIZE=350|CAPTION= <scene name='initialview01'>1a55</scene>, resolution 2.4Å | ||
|SITE= <scene name='pdbsite=2HP:Ligands+Of+2hp+(H2po4)+Ion+322'>2HP</scene> | |SITE= <scene name='pdbsite=2HP:Ligands+Of+2hp+(H2po4)+Ion+322'>2HP</scene> | ||
|LIGAND= <scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE ION'>2HP</scene> | |LIGAND= <scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE+ION'>2HP</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= PHO-S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= PHO-S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a55 OCA], [http://www.ebi.ac.uk/pdbsum/1a55 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a55 RCSB]</span> | |||
}} | }} | ||
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[[Category: Vasisht, N.]] | [[Category: Vasisht, N.]] | ||
[[Category: Webb, M R.]] | [[Category: Webb, M R.]] | ||
[[Category: phosphotransferase]] | [[Category: phosphotransferase]] | ||
[[Category: transport]] | [[Category: transport]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:33:28 2008'' | ||
Revision as of 18:33, 30 March 2008
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| , resolution 2.4Å | |||||||
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| Sites: | |||||||
| Ligands: | |||||||
| Gene: | PHO-S (Escherichia coli) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PHOSPHATE-BINDING PROTEIN MUTANT A197C
OverviewOverview
Crystal structures are presented for the A197C mutant of Escherichia coli phosphate binding protein (PBP) and the same mutant labeled at Cys197 with N-[2-(1-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Both proteins are complexed with inorganic phosphate. The latter molecule, MDCC-PBP, exhibits a large increase in fluorescence on binding inorganic phosphate. The resulting high-fluorescence state of the coumarin and the ability of this coumarin to monitor the conformational changes associated with inorganic phosphate binding are interpreted in terms of the specific interactions of MDCC with the protein. The structure helps to explain why this particular label gives a high-fluorescence state on binding inorganic phosphate, while several other related labels do not, and hence aids our general understanding of environmentally sensitive fluorescence probes on proteins.
About this StructureAbout this Structure
1A55 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate., Hirshberg M, Henrick K, Haire LL, Vasisht N, Brune M, Corrie JE, Webb MR, Biochemistry. 1998 Jul 21;37(29):10381-5. PMID:9671506
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