1a3d: Difference between revisions
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|PDB= 1a3d |SIZE=350|CAPTION= <scene name='initialview01'>1a3d</scene>, resolution 1.8Å | |PDB= 1a3d |SIZE=350|CAPTION= <scene name='initialview01'>1a3d</scene>, resolution 1.8Å | ||
|SITE= <scene name='pdbsite=CAB:Ca+Binding+Residues'>CAB</scene> | |SITE= <scene name='pdbsite=CAB:Ca+Binding+Residues'>CAB</scene> | ||
|LIGAND= <scene name='pdbligand=NA:SODIUM ION'>NA</scene> | |LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a3d OCA], [http://www.ebi.ac.uk/pdbsum/1a3d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a3d RCSB]</span> | |||
}} | }} | ||
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[[Category: Segelke, B W.]] | [[Category: Segelke, B W.]] | ||
[[Category: Xuong, H N.]] | [[Category: Xuong, H N.]] | ||
[[Category: activator site]] | [[Category: activator site]] | ||
[[Category: calcium binding]] | [[Category: calcium binding]] | ||
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[[Category: trimer]] | [[Category: trimer]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:32:22 2008'' | ||
Revision as of 18:32, 30 March 2008
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| , resolution 1.8Å | |||||||
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| Sites: | |||||||
| Ligands: | |||||||
| Activity: | Phospholipase A(2), with EC number 3.1.1.4 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PHOSPHOLIPASE A2 (PLA2) FROM NAJA NAJA VENOM
OverviewOverview
Three crystal forms of Naja naja naja phospholipase A2 were discovered through random crystallization screening, including two heretofore uncharacterized forms. The crystallization conditions for both of these novel crystal forms are Ca(2+)-free whereas previously reported conditions include Ca2+. One of the new crystal forms has a cubic lattice in the space group P2(1)3 (a = b = c = 69.24 A), the other has an orthorhombic lattice in the space group P2(1)2(1)2(1) (a = 67.22 A, b = 73.48 A, c = 87.52 A) and a previously characterized crystal belong to the tetragonal space group P4(3)2(1)2 (a = b = 88.6 A, c = 107.4 A). The structure from the cubic crystal form has been determined to 1.8 A and refined to an R-factor of 17% while the structure from the orthorhombic form has been determined to 2.65 A and has been refined to an R-factor of 21%. The determination of the cubic structure extends the resolution to which structures of this molecule have been determined from 2.3 A to 1.8 A. The two newly determined structures, in combination with the previously determined structure, generate an informative structural ensemble from which structural changes due to Ca2+, which is required for catalysis, and the effect of crystal contacts on side-chain conformations and oligomeric association can be inferred. Both of the newly determined structures reveal a trimeric oligomer as observed in the tetragonal structure; this appears to be a unique feature of the Naja naja naja enzyme.
About this StructureAbout this Structure
1A3D is a Single protein structure of sequence from Naja naja. Full crystallographic information is available from OCA.
ReferenceReference
Structures of two novel crystal forms of Naja naja naja phospholipase A2 lacking Ca2+ reveal trimeric packing., Segelke BW, Nguyen D, Chee R, Xuong NH, Dennis EA, J Mol Biol. 1998 May 29;279(1):223-32. PMID:9636712
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