2n5c: Difference between revisions
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''' | ==Solution NMR structure of the lasso peptide chaxapeptin== | ||
<StructureSection load='2n5c' size='340' side='right' caption='[[2n5c]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2n5c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_leeuwenhoekii Streptomyces leeuwenhoekii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N5C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N5C FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n5c OCA], [http://pdbe.org/2n5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n5c RCSB], [http://www.ebi.ac.uk/pdbsum/2n5c PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Lasso peptides are ribosomally synthesized and post-translationally modified peptides (RiPPs) that possess a unique "lariat knot" structural motif. Genome mining-targeted discovery of new natural products from microbes obtained from extreme environments has led to the identification of a gene cluster directing the biosynthesis of a new lasso peptide, designated as chaxapeptin 1, in the genome of Streptomyces leeuwenhoekii strain C58 isolated from the Atacama Desert. Subsequently, 1 was isolated and characterized using high-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance methods. The lasso nature of 1 was confirmed by calculating its nuclear Overhauser effect restraint-based solution structure. Chaxapeptin 1 displayed a significant inhibitory activity in a cell invasion assay with human lung cancer cell line A549. | |||
Chaxapeptin, a Lasso Peptide from Extremotolerant Streptomyces leeuwenhoekii Strain C58 from the Hyperarid Atacama Desert.,Elsayed SS, Trusch F, Deng H, Raab A, Prokes I, Busarakam K, Asenjo JA, Andrews BA, van West P, Bull AT, Goodfellow M, Yi Y, Ebel R, Jaspars M, Rateb ME J Org Chem. 2015 Sep 24. PMID:26402731<ref>PMID:26402731</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2n5c" style="background-color:#fffaf0;"></div> | |||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: Bull, A | </StructureSection> | ||
[[Category: Streptomyces leeuwenhoekii]] | |||
[[Category: Andrews, B A]] | |||
[[Category: Asenjo, J A]] | |||
[[Category: Bull, A T]] | |||
[[Category: Busarakam, K]] | [[Category: Busarakam, K]] | ||
[[Category: Elsayed, S | [[Category: Deng, H]] | ||
[[Category: Ebel, R]] | |||
[[Category: Elsayed, S S]] | |||
[[Category: Goodfellow, M]] | [[Category: Goodfellow, M]] | ||
[[Category: Jaspars, M]] | |||
[[Category: Prokes, I]] | [[Category: Prokes, I]] | ||
[[Category: | [[Category: Raab, A]] | ||
[[Category: | [[Category: Rateb, M E]] | ||
[[Category: Trusch, F]] | [[Category: Trusch, F]] | ||
[[Category: West, P van]] | |||
[[Category: Yi, Y]] | |||
[[Category: Atacama desert]] | |||
[[Category: Cell invasion]] | |||
[[Category: Chaxapeptin]] | |||
[[Category: Genome mining]] | |||
[[Category: Ripp]] | |||
[[Category: Streptomyce]] | |||
Revision as of 16:46, 7 October 2015
Solution NMR structure of the lasso peptide chaxapeptinSolution NMR structure of the lasso peptide chaxapeptin
Structural highlights
Publication Abstract from PubMedLasso peptides are ribosomally synthesized and post-translationally modified peptides (RiPPs) that possess a unique "lariat knot" structural motif. Genome mining-targeted discovery of new natural products from microbes obtained from extreme environments has led to the identification of a gene cluster directing the biosynthesis of a new lasso peptide, designated as chaxapeptin 1, in the genome of Streptomyces leeuwenhoekii strain C58 isolated from the Atacama Desert. Subsequently, 1 was isolated and characterized using high-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance methods. The lasso nature of 1 was confirmed by calculating its nuclear Overhauser effect restraint-based solution structure. Chaxapeptin 1 displayed a significant inhibitory activity in a cell invasion assay with human lung cancer cell line A549. Chaxapeptin, a Lasso Peptide from Extremotolerant Streptomyces leeuwenhoekii Strain C58 from the Hyperarid Atacama Desert.,Elsayed SS, Trusch F, Deng H, Raab A, Prokes I, Busarakam K, Asenjo JA, Andrews BA, van West P, Bull AT, Goodfellow M, Yi Y, Ebel R, Jaspars M, Rateb ME J Org Chem. 2015 Sep 24. PMID:26402731[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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