2pc6: Difference between revisions

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Revision as of 07:39, 26 March 2008

File:2pc6.gif

, resolution 2.50Å

Ligands:

, ,

Gene:

ilvH, NE1324 (Nitrosomonas europaea ATCC 19718)

Activity:

Acetolactate synthase, with EC number 2.2.1.6

Domains:

ilvH, ilvH, ACT_AHAS

Resources:

FirstGlance, OCA, PDBsum, JenaLib, RCSB

Coordinates:

save as pdb, mmCIF, xml

Crystal structure of putative acetolactate synthase- small subunit from Nitrosomonas europaea

OverviewOverview

Crystal structures of two orthologs of the regulatory subunit of acetohydroxyacid synthase III (AHAS, EC 2.2.1.6) from Thermotoga maritima (TM0549) and Nitrosomonas europea (NE1324) were determined by single-wavelength anomalous diffraction methods with the use of selenomethionine derivatives at 2.3 A and 2.5 A, respectively. TM0549 and NE1324 share the same fold, and in both proteins the polypeptide chain contains two separate domains of a similar size. Each protein contains a C-terminal domain with ferredoxin-type fold and an N-terminal ACT domain, of which the latter is characteristic for several proteins involved in amino acid metabolism. The ferredoxin domain is stabilized by a calcium ion in the crystal structure of NE1324 and by a Mg(H2O)(6)2+ ion in TM0549. Both TM0549 and NE1324 form dimeric assemblies in the crystal lattice.

About this StructureAbout this Structure

2PC6 is a Single protein structure of sequence from Nitrosomonas europaea atcc 19718. This structure supersedes the now removed PDB entry 2FGD. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit., Petkowski JJ, Chruszcz M, Zimmerman MD, Zheng H, Skarina T, Onopriyenko O, Cymborowski MT, Koclega KD, Savchenko A, Edwards A, Minor W, Protein Sci. 2007 Jul;16(7):1360-7. PMID:17586771

Page seeded by OCA on Wed Mar 26 06:39:16 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 4: / Line 4: @@
 |PDB= 2pc6 |SIZE=350|CAPTION= <scene name='initialview01'>2pc6</scene>, resolution 2.50&Aring;
 |SITE=
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=SIN:SUCCINIC ACID'>SIN</scene>
+|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene>
-|ACTIVITY= [http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6]
+|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span>
 |GENE= ilvH, NE1324 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228410 Nitrosomonas europaea ATCC 19718])
+|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK11895 ilvH], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=CHL00100 ilvH], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd04878 ACT_AHAS]</span>
+|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pc6 OCA], [http://www.ebi.ac.uk/pdbsum/2pc6 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=2pc6 RCSB]</span>
 }}
@@ Line 35: / Line 37: @@
 [[Category: Zheng, H.]]
 [[Category: Zimmerman, M D.]]
-[[Category: CA]]
-[[Category: SIN]]
 [[Category: acetolactate synthase]]
 [[Category: lyase]]
@@ Line 46: / Line 46: @@
 [[Category: structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:11:25 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:39:16 2008''