2p35: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 2p35 |SIZE=350|CAPTION= <scene name='initialview01'>2p35</scene>, resolution 1.95Å | |PDB= 2p35 |SIZE=350|CAPTION= <scene name='initialview01'>2p35</scene>, resolution 1.95Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Trans-aconitate_2-methyltransferase Trans-aconitate 2-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.144 2.1.1.144] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trans-aconitate_2-methyltransferase Trans-aconitate 2-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.144 2.1.1.144] </span> | ||
|GENE= tam, Atu0870, AGR_C_1589 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176299 Agrobacterium tumefaciens str. C58]) | |GENE= tam, Atu0870, AGR_C_1589 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176299 Agrobacterium tumefaciens str. C58]) | ||
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam08241 Methyltransf_11], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG4106 Tam]</span> | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p35 OCA], [http://www.ebi.ac.uk/pdbsum/2p35 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=2p35 RCSB]</span> | |||
}} | }} | ||
| Line 30: | Line 32: | ||
[[Category: Xu, X.]] | [[Category: Xu, X.]] | ||
[[Category: Zheng, H.]] | [[Category: Zheng, H.]] | ||
[[Category: agrobacterium tumefacien]] | [[Category: agrobacterium tumefacien]] | ||
[[Category: mcsg]] | [[Category: mcsg]] | ||
| Line 40: | Line 41: | ||
[[Category: trans-aconitate methyltransferase]] | [[Category: trans-aconitate methyltransferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:38:25 2008'' | ||
Revision as of 07:38, 26 March 2008
| |||||||
| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | tam, Atu0870, AGR_C_1589 (Agrobacterium tumefaciens str. C58) | ||||||
| Activity: | Trans-aconitate 2-methyltransferase, with EC number 2.1.1.144 | ||||||
| Domains: | Methyltransf_11, Tam | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of trans-aconitate methyltransferase from Agrobacterium tumefaciens
OverviewOverview
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
About this StructureAbout this Structure
2P35 is a Single protein structure of sequence from Agrobacterium tumefaciens str. c58. Full crystallographic information is available from OCA.
ReferenceReference
In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461
Page seeded by OCA on Wed Mar 26 06:38:25 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Agrobacterium tumefaciens str. c58
- Single protein
- Trans-aconitate 2-methyltransferase
- Chang, C.
- Edwards, A M.
- Joachimiak, A.
- MCSG, Midwest Center for Structural Genomics.
- Savchenko, A.
- Xu, X.
- Zheng, H.
- Agrobacterium tumefacien
- Mcsg
- Midwest center for structural genomic
- Protein structure initiative
- Psi-2
- Sam dependent methyltransferase
- Structural genomic
- Trans-aconitate methyltransferase