Cas9 Sandbox: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Brett M. Thumm, Ann Taylor, Sam Hayes, Justin Woodard

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-==Your Heading Here (maybe something like 'Structure')==
+[[Cas9]] is the  RNA-guided [[DNA]] [[endonuclease]] used by the CRISPR (clustered regularly interspaced short palindromic repeats)-associated systems to generate double-strand DNA breaks in the invading DNA during an adaptive bacterial immune response.
-<StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''>
-This is a default text for your page '''Cas9 Sandbox'''. Click above on '''edit this page''' to modify. Be careful with the &lt; and &gt; signs.
+<Structure load='Insert PDB code or filename here' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />
-You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.
+The CRISPR-associated endonuclease [[Cas9]] has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences.
+[[Image:Layout for schematic and structure with structure.png|660px]]
+Articles in Proteopedia concerning Cas9 include:
+* any?
+==3D structures of Cas9==
+===''Streptococcus pyogenes'' Cas9===
+* [[4un3]], [[4un4]], and [[4un5]] -  ''S. pyogenes'' Cas9 bound to sgRNA and target DNA
+[[Image:4un3 labeled.png|right|390px]]
+* [[4oo8]] -  ''S. pyogenes'' Cas9 bound to sgRNA and target DNA
+* [[4cmp]]
+* [[4cmq]]  - Mn<sup>2+</sup>-bound ''S. pyogenes'' Cas9
+=== ''Actinomyces naeslundii'' Cas9===
+*[[4oge]]
+*[[4ogc]]  - Mn<sup>2+</sup>-bound ''A.s naeslundii'' Cas9
+==See Also==
+[[HNH endonuclease]] <br/>
-== Function ==
-<Structure load='Insert PDB code or filename here' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />
-== Disease ==
+== References ==
+<references/>
+[[Category: Crispr]]
+[[Category: Crispr-associated]]
+[[Category: endonuclease]]
+The CRISPR-associated endonuclease [[Cas9]] has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences.
+[[Image:Layout for schematic and structure with structure.png|660px]]
+Articles in Proteopedia concerning Cas9 include:
+* any?
+==3D structures of Cas9==
+===''Streptococcus pyogenes'' Cas9===
+* [[4un3]], [[4un4]], and [[4un5]] -  ''S. pyogenes'' Cas9 bound to sgRNA and target DNA
+[[Image:4un3 labeled.png|right|390px]]
+* [[4oo8]] -  ''S. pyogenes'' Cas9 bound to sgRNA and target DNA
+* [[4cmp]]
+* [[4cmq]]  - Mn<sup>2+</sup>-bound ''S. pyogenes'' Cas9
+=== ''Actinomyces naeslundii'' Cas9===
+*[[4oge]]
+*[[4ogc]]  - Mn<sup>2+</sup>-bound ''A.s naeslundii'' Cas9
-== Relevance ==
+==See Also==
+[[HNH endonuclease]] <br/>
-== Structural highlights ==
-This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
-</StructureSection>
 == References ==
 <references/>
+[[Category: Crispr]]
+[[Category: Crispr-associated]]
+[[Category: endonuclease]]