2r6f: Difference between revisions
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|PDB= 2r6f |SIZE=350|CAPTION= <scene name='initialview01'>2r6f</scene>, resolution 3.200Å | |PDB= 2r6f |SIZE=350|CAPTION= <scene name='initialview01'>2r6f</scene>, resolution 3.200Å | ||
|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+A+1004'>AC1</scene>, <scene name='pdbsite=AC2:Zn+Binding+Site+For+Residue+A+1005'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Residue+A+1006'>AC3</scene>, <scene name='pdbsite=AC4:Zn+Binding+Site+For+Residue+B+1007'>AC4</scene>, <scene name='pdbsite=AC5:Zn+Binding+Site+For+Residue+B+1008'>AC5</scene>, <scene name='pdbsite=AC6:Zn+Binding+Site+For+Residue+B+1009'>AC6</scene>, <scene name='pdbsite=AC7:Adp+Binding+Site+For+Residue+A+1000'>AC7</scene>, <scene name='pdbsite=AC8:Adp+Binding+Site+For+Residue+A+1001'>AC8</scene>, <scene name='pdbsite=AC9:Adp+Binding+Site+For+Residue+B+1002'>AC9</scene> and <scene name='pdbsite=BC1:Adp+Binding+Site+For+Residue+B+1003'>BC1</scene> | |SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+A+1004'>AC1</scene>, <scene name='pdbsite=AC2:Zn+Binding+Site+For+Residue+A+1005'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Residue+A+1006'>AC3</scene>, <scene name='pdbsite=AC4:Zn+Binding+Site+For+Residue+B+1007'>AC4</scene>, <scene name='pdbsite=AC5:Zn+Binding+Site+For+Residue+B+1008'>AC5</scene>, <scene name='pdbsite=AC6:Zn+Binding+Site+For+Residue+B+1009'>AC6</scene>, <scene name='pdbsite=AC7:Adp+Binding+Site+For+Residue+A+1000'>AC7</scene>, <scene name='pdbsite=AC8:Adp+Binding+Site+For+Residue+A+1001'>AC8</scene>, <scene name='pdbsite=AC9:Adp+Binding+Site+For+Residue+B+1002'>AC9</scene> and <scene name='pdbsite=BC1:Adp+Binding+Site+For+Residue+B+1003'>BC1</scene> | ||
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=ADP:ADENOSINE-5 | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= uvrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus]) | |GENE= uvrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus]) | ||
| Line 45: | Line 45: | ||
[[Category: zinc]] | [[Category: zinc]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:48:04 2008'' | ||
Revision as of 16:48, 23 March 2008
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| , resolution 3.200Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , , , and | ||||||
| Ligands: | and | ||||||
| Gene: | uvrA (Geobacillus stearothermophilus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Bacillus stearothermophilus UvrA
OverviewOverview
The nucleotide excision repair pathway corrects many structurally unrelated DNA lesions. Damage recognition in bacteria is performed by UvrA, a member of the ABC ATPase superfamily whose functional form is a dimer with four nucleotide-binding domains (NBDs), two per protomer. In the 3.2 A structure of UvrA from Bacillus stearothermophilus, we observe that the nucleotide-binding sites are formed in an intramolecular fashion and are not at the dimer interface as is typically found in other ABC ATPases. UvrA also harbors two unique domains; we show that one of these is required for interaction with UvrB, its partner in lesion recognition. In addition, UvrA contains three zinc modules, the number and ligand sphere of which differ from previously published models. Structural analysis, biochemical experiments, surface electrostatics, and sequence conservation form the basis for models of ATP-modulated dimerization, UvrA-UvrB interaction, and DNA binding during the search for lesions.
About this StructureAbout this Structure
2R6F is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of Bacillus stearothermophilus UvrA provides insight into ATP-modulated dimerization, UvrB interaction, and DNA binding., Pakotiprapha D, Inuzuka Y, Bowman BR, Moolenaar GF, Goosen N, Jeruzalmi D, Verdine GL, Mol Cell. 2008 Jan 18;29(1):122-33. Epub 2007 Dec 27. PMID:18158267
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OCA- Pages with broken file links
- Geobacillus stearothermophilus
- Single protein
- Bowman, B R.
- Inuzuka, Y.
- Jeruzalmi, D.
- Pakotiprapha, D.
- Verdine, G L.
- ADP
- ZN
- Abc atpase
- Atp-binding cassette
- Cytoplasm
- Dna damage
- Dna excision
- Dna repair
- Dna-binding
- Excision nuclease
- Hydrolase
- Metal-binding
- Nucleotide excision repair
- Nucleotide-binding
- Sos response
- Uvra
- Zinc