2j4r: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 2j4r |SIZE=350|CAPTION= <scene name='initialview01'>2j4r</scene>, resolution 2.71Å | |PDB= 2j4r |SIZE=350|CAPTION= <scene name='initialview01'>2j4r</scene>, resolution 2.71Å | ||
|SITE= <scene name='pdbsite=AC1:G4p+Binding+Site+For+Chain+A'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:G4p+Binding+Site+For+Chain+A'>AC1</scene> | ||
|LIGAND= <scene name='pdbligand=G4P:GUANOSINE-5 | |LIGAND= <scene name='pdbligand=G4P:GUANOSINE-5',3'-TETRAPHOSPHATE'>G4P</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| Line 31: | Line 31: | ||
[[Category: stringent response]] | [[Category: stringent response]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:24:18 2008'' | ||
Revision as of 16:24, 23 March 2008
| |||||||
| , resolution 2.71Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURAL STUDY OF THE AQUIFEX AEOLICUS PPX-GPPA ENZYME
OverviewOverview
The crystal structure of the prototype exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family member from Aquifex aeolicus in complex with the intracellular second messenger guanosine tetraphosphate was determined at 2.7-A resolution. The hydrolytic base is identified as E119. The dual specificity established for the Escherichia coli homolog is shown to be compatible with a common active site for guanosine pentaphosphate and polyphosphate hydrolysis. Distinct and different degrees of closure between the two domains of the enzyme are associated with substrate binding. The arginines R22 and R267, residing in different domains, are crucial for guanosine pentaphosphate specificity as they interact with the unique 3'-ribose phosphorylation.
About this StructureAbout this Structure
2J4R is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the PPX/GPPA phosphatase from Aquifex aeolicus in complex with the alarmone ppGpp., Kristensen O, Ross B, Gajhede M, J Mol Biol. 2008 Feb 1;375(5):1469-76. Epub 2007 Dec 4. PMID:18155044
Page seeded by OCA on Sun Mar 23 15:24:18 2008