2bt1: Difference between revisions
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|PDB= 2bt1 |SIZE=350|CAPTION= <scene name='initialview01'>2bt1</scene>, resolution 2.70Å | |PDB= 2bt1 |SIZE=350|CAPTION= <scene name='initialview01'>2bt1</scene>, resolution 2.70Å | ||
|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene> | ||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=DUP:2 | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=DUP:2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE'>DUP</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] | |ACTIVITY= [http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] | ||
|GENE= | |GENE= | ||
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[[Category: structural proteomics in europe]] | [[Category: structural proteomics in europe]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 14:47:39 2008'' | ||
Revision as of 15:47, 23 March 2008
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| , resolution 2.70Å | |||||||
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| Sites: | |||||||
| Ligands: | and | ||||||
| Activity: | dUTP diphosphatase, with EC number 3.6.1.23 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
EPSTEIN BARR VIRUS DUTPASE IN COMPLEX WITH A,B-IMINO DUTP
OverviewOverview
Deoxyuridine 5'-triphosphate pyrophosphatases (dUTPases) are ubiquitous enzymes cleaving dUTP into dUMP and pyrophosphate. They occur as monomeric, dimeric, or trimeric molecules. The trimeric and monomeric enzymes both contain the same five characteristic sequence motifs but in a different order, whereas the dimeric enzymes are not homologous. Monomeric dUTPases only occur in herpesviruses, such as Epstein-Barr virus (EBV). Here, we describe the crystal structures of EBV dUTPase in complex with the product dUMP and a substrate analog alpha,beta-imino-dUTP. The molecule consists of three domains forming one active site that has a structure extremely similar to one of the three active sites of trimeric dUTPases. The three domains functionally correspond to the subunits of the trimeric form. Domains I and II have the dUTPase fold, but they differ considerably in the regions that are not involved in the formation of the unique active site, whereas domain III has only little secondary structure.
About this StructureAbout this Structure
2BT1 is a Single protein structure of sequence from Human herpesvirus 4. Full crystallographic information is available from OCA.
ReferenceReference
The monomeric dUTPase from Epstein-Barr virus mimics trimeric dUTPases., Tarbouriech N, Buisson M, Seigneurin JM, Cusack S, Burmeister WP, Structure. 2005 Sep;13(9):1299-310. PMID:16154087
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