1umq: Difference between revisions
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<StructureSection load='1umq' size='340' side='right' caption='[[1umq]], [[NMR_Ensembles_of_Models | 5 NMR models]]' scene=''> | <StructureSection load='1umq' size='340' side='right' caption='[[1umq]], [[NMR_Ensembles_of_Models | 5 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1umq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[1umq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhodococcus_capsulatus"_molisch_1907 "rhodococcus capsulatus" molisch 1907]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UMQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UMQ FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1umq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1umq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1umq RCSB], [http://www.ebi.ac.uk/pdbsum/1umq PDBsum]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1umq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1umq OCA], [http://pdbe.org/1umq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1umq RCSB], [http://www.ebi.ac.uk/pdbsum/1umq PDBsum]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 1umq" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Rhodococcus capsulatus molisch 1907]] | ||
[[Category: Laguri, C]] | [[Category: Laguri, C]] | ||
[[Category: Phillips-Jones, M K]] | [[Category: Phillips-Jones, M K]] | ||
Revision as of 13:50, 10 September 2015
SOLUTION STRUCTURE AND DNA BINDING OF THE EFFECTOR DOMAIN FROM THE GLOBAL REGULATOR PRRA(REGA) FROM R. SPHAEROIDES: INSIGHTS INTO DNA BINDING SPECIFICITYSOLUTION STRUCTURE AND DNA BINDING OF THE EFFECTOR DOMAIN FROM THE GLOBAL REGULATOR PRRA(REGA) FROM R. SPHAEROIDES: INSIGHTS INTO DNA BINDING SPECIFICITY
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedPrr/RegA response regulator is a global transcription regulator in purple bacteria Rhodobacter sphaeroides and Rhodobacter capsulatus, and is essential in controlling the metabolic changes between aerobic and anaerobic environments. We report here the structure determination by NMR of the C-terminal effector domain of PrrA, PrrAC. It forms a three-helix bundle containing a helix-turn-helix DNA binding motif. The fold is similar to FIS protein, but the domain architecture is different from previously characterised response regulator effector domains, as it is shorter than any characterised so far. Alignment of Prr/RegA DNA targets permitted a refinement of the consensus sequence, which contains two GCGNC inverted repeats with variable half-site spacings. NMR titrations of PrrAC with specific and non-specific DNA show which surfaces are involved in DNA binding and suggest residues important for binding specificity. A model of the PrrAC/DNA complex was constructed in which two PrrAC molecules are bound to DNA in a symmetrical manner. Solution structure and DNA binding of the effector domain from the global regulator PrrA (RegA) from Rhodobacter sphaeroides: insights into DNA binding specificity.,Laguri C, Phillips-Jones MK, Williamson MP Nucleic Acids Res. 2003 Dec 1;31(23):6778-87. PMID:14627811[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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