1t1b: Difference between revisions
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|PDB= 1t1b |SIZE=350|CAPTION= <scene name='initialview01'>1t1b</scene>, resolution 1.60Å | |PDB= 1t1b |SIZE=350|CAPTION= <scene name='initialview01'>1t1b</scene>, resolution 1.60Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=HC4:4 | |LIGAND= <scene name='pdbligand=HC4:4'-HYDROXYCINNAMIC ACID'>HC4</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila]) | |GENE= PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila]) | ||
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[[Category: photoactive yellow protein]] | [[Category: photoactive yellow protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 13:40:48 2008'' | ||
Revision as of 14:40, 23 March 2008
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| , resolution 1.60Å | |||||||
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| Ligands: | |||||||
| Gene: | PYP (Halorhodospira halophila) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Late intermediate IL2 from time-resolved crystallography of the E46Q mutant of PYP
OverviewOverview
In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational change in the protein associated with differential signaling activity, as it executes a reversible photocycle. Time-resolved Laue crystallography allows structural snapshots (as short as 150 ps) of high crystallographic resolution (approximately 1.6 A) to be taken of a protein as it functions. Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns-100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion. A clear structural progression is visible in these intermediates, in which a signal generated at the chromophore propagates through a distinct structural pathway of conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore.
About this StructureAbout this Structure
1T1B is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.
ReferenceReference
A structural pathway for signaling in the E46Q mutant of photoactive yellow protein., Rajagopal S, Anderson S, Srajer V, Schmidt M, Pahl R, Moffat K, Structure. 2005 Jan;13(1):55-63. PMID:15642261
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