1s8f: Difference between revisions
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|PDB= 1s8f |SIZE=350|CAPTION= <scene name='initialview01'>1s8f</scene>, resolution 1.77Å | |PDB= 1s8f |SIZE=350|CAPTION= <scene name='initialview01'>1s8f</scene>, resolution 1.77Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GDP:GUANOSINE-5 | |LIGAND= <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene> and <scene name='pdbligand=BEZ:BENZOIC ACID'>BEZ</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= RAB9A, RAB9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9615 Canis lupus familiaris]) | |GENE= RAB9A, RAB9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9615 Canis lupus familiaris]) | ||
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[[Category: vesicular trafficking]] | [[Category: vesicular trafficking]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 13:34:53 2008'' | ||
Revision as of 14:34, 23 March 2008
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| , resolution 1.77Å | |||||||
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| Ligands: | , , , and | ||||||
| Gene: | RAB9A, RAB9 (Canis lupus familiaris) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II
OverviewOverview
The small GTPase Rab9 is an essential regulator of vesicular transport from the late endosome to the trans-Golgi network, as monitored by the redirection of the mannose-6-phosphate receptors. The crystal structure of Rab9 complexed to GDP, Mg(2+), and Sr(2+) reveals a unique dimer formed by an intermolecular beta-sheet that buries the switch I regions. Surface area and shape complementarity calculations suggest that Rab9 dimers can form an inactive, membrane-bound pool of Rab9 . GDP that is independent of GDI. Mg(2+)-bound Rab9 represents an inactive state, but Sr(2+)-bound Rab9 . GDP displays activated switch region conformations, mimicking those of the GTP state. A hydrophobic tetrad is formed resembling an effector-discriminating epitope found only in GTP-bound Rab proteins.
About this StructureAbout this Structure
1S8F is a Single protein structure of sequence from Canis lupus familiaris. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II., Wittmann JG, Rudolph MG, FEBS Lett. 2004 Jun 18;568(1-3):23-9. PMID:15196914
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